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Ante-mortem diagnosis of caprine tuberculosis in persistently infected herds: Influence of lesion type on the sensitivity of diagnostic tests

Research in Veterinary Science publish this investigation article

December 1st, 2013

Caprine tuberculosis is a major health problem for goats, and an important zoonosis. Eradication programmes using the comparative skin test are being developed to aid in decreasing the prevalence of infection. However, persistent tuberculosis infections are found in herds subjected to eradication programmes. In the present study a commercial IFN-γ release assay and an experimental ELISA based on MPB70, were evaluated as potential ancillary tests to detect infection. The relationship between the three techniques (skin test, IFN-γ release and ELISA) and histopathological lesions was analyzed in 162 goats from herds with persistent tuberculosis infection. The presence of related pathogens (paratuberculosis and pseudotuberculosis) was also studied. The IFN-γ release assay and the ELISA had a higher sensitivity than the comparative skin test (65.3% and 66.3% vs 44.5%) using as a gold standard a combination of histopathological analysis and isolation. Used in combination, ELISA and the skin test detected 89.1% of goats with tuberculosis while a combination of IFN-γ assay and skin test detected 78.2%. The types of macroscopic and microscopic lesions reflected the results of diagnostic tests




Buendia AJ., Navarro JA., Salinas J., McNair J., de Juan L., Ortega N., Camara P., Torreblanca P. and Sanchez J.




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Ante-mortem diagnosis of caprine tuberculosis in persistently infected herds: Influence of lesion type on the sensitivity of diagnostic tests

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Ante-mortem diagnosis of caprine tuberculosis in persistently infected herds: Influence of lesion type on the sensitivity of diagnostic tests


Participants:

Universidad de MurciaFacultad de Veterinaria. Universidad de Murcia (UM).

Veterinary Sciences Division (VSD). Agri-Food and Biosciences Institute (AFBI).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Comunidad Autónoma de la Región de MurciaServicio de Sanidad Animal. Consejería de Agricultura y Agua (CAA). Comunidad Autónoma de la Región de Murcia (CARM).







Research in Veterinary Science
FACTOR YEAR Q
1.511 2013

NLMID: 401300

PMID: 24183285

ISSN: 0034-5288



TITLE: Ante-mortem diagnosis of caprine tuberculosis in persistently infected herds: Influence of lesion type on the sensitivity of diagnostic tests


JOURNAL: Res. Vet. Sci.


NUMERACIÓN: 95(3):1107-13


AÑO: 2013


PUBLISHER: Elsevier


AUTHORS: Buendia AJ., Navarro JA., Salinas J., McNair J., de Juan L., Ortega N., Camara P., Torreblanca P. and Sanchez J.


VISAVET PARTICIPANTS


Lucía de Juan Ferré

DOI: https://doi.org/ 10.1016/j.rvsc.2013.10.003


CITE THIS PUBLICATION:

Buendia AJ., Navarro JA., Salinas J., McNair J., de Juan L., Ortega N., Camara P., Torreblanca P. and Sanchez J. Ante-mortem diagnosis of caprine tuberculosis in persistently infected herds: Influence of lesion type on the sensitivity of diagnostic tests. Research in Veterinary Science. 95(3):1107-13. 2013. (A). ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2013.10.003


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