Investigation published in Antimicrobial Agents and Chemotherapy

May 1^st, 2015

We describe the genetic background of blaTEM-4 and the complete sequence of pRYC11::blaTEM-4, a mosaic plasmid highly similar to pKpQIL-like variants, predominant among TEM-4 producers in a Spanish hospital (1990-2004) which belong to Klebsiella pneumoniae and Escherichia coli high-risk clones responsible for the current spread of different antibiotic resistance genes. Predominant populations of plasmids and host adapted clonal lineages seem to have greatly contributed to the spread of resistance to extended-spectrum cephalosporins

Rodriguez I., Novais A., Lira F., Valverde A., Curiao T., Martinez JL., Baquero F., Canton R. and Coque TM.

Antibiotic-resistant Klebsiella pneumoniae and Escherichia coli high-risk clones and an IncFII(k) mosaic plasmid hosting Tn1 (blaTEM-4) in isolates from 1990 to 2004

Antibiotic-resistant Klebsiella pneumoniae and Escherichia coli high-risk clones and an IncFII(k) mosaic plasmid hosting Tn1 (blaTEM-4) in isolates from 1990 to 2004

	Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP). Instituto de Salud Carlos III (ISCIII).
	Centro de Investigação em Biodiversidade e Recursos Genétivos (CIBIO). Universidade do Porto (UP).
	Centro Nacional de Biotecnología (CNB). Consejo Superior de Investigaciones Científicas (CSIC).
	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Red Española de Investigación en Patología Infecciosa (REIPI). Instituto de Salud Carlos III (ISCIII).
	Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.