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Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts

Applied and Environmental Microbiology publish this investigation article

December 1st, 1992

We studied the usefulness of an immunoblot technique for the detection of staphylococcal enterotoxins (SEs) in strains and food extracts. Food samples (milk, yogurt, hot dog sausage, cheese, and mayonnaise) were artificially contaminated with SEA through SEE. Protein A did not interfere with the results; it appeared on electrophoresis gels as bands with molecular weights higher than those of the SEs. Other food proteins were not revealed by the technique. The immunoblot technique proved to be fast, specific, and sensitive for the detection of SEs in foods




Orden JA., Goyache J., Hernandez J., Domenech A., Suarez G. and Gomez-Lucia E.




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Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts

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Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Applied and Environmental Microbiology
FACTOR YEAR Q
3.336 1997

NLMID: 7605801

PMID: 1476449

ISSN: 0099-2240



TITLE: Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts


JOURNAL: Appl Environ Microbiol


NUMERACIÓN: 58(12):4083-5


AÑO: 1992


PUBLISHER: American Society for Microbiology


AUTHORS: Orden JA., Goyache J., Hernandez J., Domenech A., Suarez G. and Gomez-Lucia E.


Joaquín Goyache Goñi

DOI: https://doi.org/10.1128/AEM.58.12.4083-4085.1992


CITE THIS PUBLICATION:

Orden JA., Goyache J., Hernandez J., Domenech A., Suarez G. and Gomez-Lucia E. Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts. Applied and Environmental Microbiology. 58(12):4083-5. 1992. (A). ISSN: 0099-2240. DOI: 10.1128/AEM.58.12.4083-4085.1992


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