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Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK

Investigation published in The Journal of Antimicrobial Chemotherapy

July 1st, 2013

OBJECTIVES: 16S rRNA methyltransferases are an emerging mechanism conferring high-level resistance to clinically relevant aminoglycosides and have been associated with important mechanisms such as NDM-1. We sought genes encoding these enzymes in isolates highly resistant (MIC >200 mg/L) to gentamicin and amikacin from an Indian hospital and we additionally screened for the novel RmtF enzyme in 132 UK isolates containing NDM. METHODS: All highly aminoglycoside-resistant isolates were screened for armA and rmtA-E by PCR, with cloning experiments performed for isolates negative for these genes. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry was used to determine the methylation target of the novel RmtF methyltransferase. RmtF-bearing strains were characterized further, including susceptibility testing, PFGE, electroporation, PCR-based replicon typing and multilocus sequence typing of rmtF-bearing plasmids. RESULTS: High-level aminoglycoside resistance was detected in 140/1000 (14%) consecutive isolates of Enterobacteriaceae from India. ArmA, RmtB and RmtC were identified among 46%, 20% and 27% of these isolates, respectively. The novel rmtF gene was detected in 34 aminoglycoside-resistant isolates (overall prevalence 3.4%), most (59%) of which also possessed a blaNDM gene; rmtF was detected in 6 NDM producers from the UK. It was found on different plasmid backbones. Four and two isolates showed resistance to tigecycline and colistin, respectively. CONCLUSIONS: RmtF was often found in association with NDM in members of the Enterobacteriaceae and on diverse plasmids. It is of clinical concern that the RmtF- and NDM-positive strains identified here show additional resistance to tigecycline and colistin, current drugs of last resort for the treatment of serious bacterial infections




Hidalgo L., Hopkins KL., Gutierrez B., Ovejero CM., Shukla S., Douthwaite S., Prasad KN., Woodford N. and Gonzalez-Zorn B..




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Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK

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Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK


Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Public Health EnglandAntimicrobial Resistance and Healthcare Associated Infections (AMRHAI). Public Health England (PHE).

Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS).

University of Southern DenmarkDepartment of Biochemistry and Molecular Biology. University of Southern Denmark (SDU).







The Journal of Antimicrobial Chemotherapy
FACTOR YEAR Q
5.439 2013

NLMID: 7513617

PMID: 23580560

ISSN: 0305-7453



TITLE: Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK


JOURNAL: J. Antimicrob. Chemother.


NUMERACIÓN: 68(7):1543-50


AÑO: 2013


PUBLISHER: Oxford University Press


AUTHORS: Hidalgo L., Hopkins KL., Gutierrez B., Ovejero CM., Shukla S., Douthwaite S., Prasad KN., Woodford N. and Gonzalez-Zorn B..


VISAVET PARTICIPANTS


Bruno González Zorn

DOI: https://doi.org/ 10.1093/jac/dkt078


CITE THIS PUBLICATION:

Hidalgo L., Hopkins KL., Gutierrez B., Ovejero CM., Shukla S., Douthwaite S., Prasad KN., Woodford N. and Gonzalez-Zorn B. Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK. The Journal of Antimicrobial Chemotherapy. 68(7):1543-50. 2013. (A). ISSN: 0305-7453. DOI: 10.1093/jac/dkt078


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