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Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain

Investigation article published in Veterinary Microbiology

May 15th, 2021

This study compares the utility of a commercially available multiplex q-PCR assay for serotyping A1, A2, and A6 M. haemolytica serotypes with indirect hemagglutination, for determining the relative distribution of M. haemolytica capsular types associated with respiratory disorders in cattle, sheep, and goats. For the 129 isolates analyzed, both q-PCR and IHA assays exhibited nearly complete agreement for capsular types A1 (k = 0.965) and A2 (k = 0.888) and substantial agreement for A6 (k = 0.801). Despite the overall good performance of the commercial q-PCR, its effectiveness differed between the host origin of the isolates. The serotype was identified by q-PCR in 83.3 % of cattle, 77.8 % of goat, and 53.8 % of sheep isolates. Combining the results of both methods, A1 was the most prevalent in cattle and sheep (55.6 % and 22.25 %, respectively) but was not detected in goats, A2 was the most prevalent in goats (61.1 %) and the second most prevalent in cattle (16.7 %) and sheep (20.5 %). The prevalence of A6 was 7.4 %, 5.1 %, and 16.7 % in cattle, sheep, and goats, respectively. Other capsular types determined exclusively by IHA were A16 in cattle, A9 in goats, and A7, A8, A9, and A13 in sheep. Capsular type diversity was greater in sheep (H = 0.601) than in cattle (H = 0.408) and goat (H = 0.330) isolates. The commercial multiplex q-PCR is a valuable tool, alternative to IHA, for identifying isolates of capsular types A1, A2, and A6, the most frequent serotypes of M. haemolytica associated with respiratory disease in ruminants. However, when testing sheep isolates it should be complemented with immunological assays due to the wider range of serotypes implicated




Arnal JL., Fernandez A., Vela AI., Sanz C., Fernandez-Garayzabal JF. and Cid D.




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Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain

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Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain



Participants:

Laboratorio ExopolVeterinary Diagnostic and Autogenous Vaccine Laboratory. Laboratorio Exopol.

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
3.293 2020

NLMID: 7705469

PMID: 34020174

ISSN: 0378-1135



TITLE: Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 258:109121


AÑO: 2021


PUBLISHER: Elsevier Science Publishers


AUTHORS: Arnal JL., Fernandez A., Vela AI., Sanz C., Fernandez-Garayzabal JF. and Cid D.


VISAVET PARTICIPANTS


3rd
Ana Isabel Vela Alonso
5th
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1016/j.vetmic.2021.109121


CITE THIS PUBLICATION:

Arnal JL., Fernandez A., Vela AI., Sanz C., Fernandez-Garayzabal JF. and Cid D. Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain. Veterinary Microbiology. 258:109121. 2021. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2021.109121


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