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Characterization of Leishmania infantum strains by PCR-RFLP in wild Leporidae from the Community of Madrid

Poster presented in 6th World Congress on Leishmaniasis

May 19th, 2017

Ortega-García MV., Moreno I., Garcia N., Martin AB., Navarro A., Martinez I., Garcia-Seco T., Perez-Sancho M. and Dominguez M.

Leishmania infantum is the main species implicated in Leishmaniosis in the Mediterranean basin, where the disease is endemic. Canines are the main domestic reservoirs. Recently, Leporidae have been revealed as competent reservoirs of L. infantum, being the source of infection during an important outbreak in Madrid (Spain) that involved 697 human cases from 2009 to 2016. L. infantun DNA has been detected by quantitative real-time PCR in wild rabbits and hares in two Madrid natural areas outside the outbreak area. Comparison of the strains isolated from different areas could add more knowledge about the sylvatic cycle of Leishmania spp. and PCR-RFLP can be used as a cost-effective and reliable method to achieve it.
The aims of this study are a) to characterize the L. infantum strains detected in wild Leporidae from different areas of the Community of Madrid (outbreak and non-outbreak areas); and b), to know if the L. infantum strains maintained by wild Leporidae in the outbreak area are the same as those ones found in the non-outbreak areas




Participants:

Ministerio de DefensaCampus La Marañosa (ITM). Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

Ministerio de DefensaSubdirección de Sistemas Terrestres. Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).


Link to 6th World Congress on Leishmaniasis





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6th World Congress on Leishmaniasis


6th World Congress on Leishmaniasis
May 16th-20th, 2017

TITLE: Characterization of Leishmania infantum strains by PCR-RFLP in wild Leporidae from the Community of Madrid


TYPE: Poster communication


AUTHORS: Ortega-García MV., Moreno I., Garcia N., Martin AB., Navarro A., Martinez I., Garcia-Seco T., Perez-Sancho M. and Dominguez M.


3rd
Nerea García Benzaquén
5th
Alejandro Navarro Gómez
6th
Irene Martínez Alares
7th
María Teresa García-Seco Romero
8th
Marta Pérez Sancho
Last
Mercedes Domínguez Rodríguez

DATE: May 19th, 2017


CITE THIS COMMUNICATION:

Ortega-García MV., Moreno I., Garcia N., Martin AB., Navarro A., Martinez I., Garcia-Seco T., Perez-Sancho M. and Dominguez M. Characterization of Leishmania infantum strains by PCR-RFLP in wild Leporidae from the Community of Madrid. 6th World Congress on Leishmaniasis, Instituto de Salud Carlos III, Drugs for Neglected Diseases initiative, May 19th, 2017. (Poster communication)


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