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Characterization of plasmids in a human clinical strain of Lactococcus garvieae

Investigation article published in PLoS ONE

June 29th, 2012

The present work describes the molecular characterization of five circular plasmids found in the human clinical strain Lactococcus garvieae 21881. The plasmids were designated pGL1-pGL5, with molecular sizes of 4,536 bp, 4,572 bp, 12,948 bp, 14,006 bp and 68,798 bp, respectively. Based on detailed sequence analysis, some of these plasmids appear to be mosaics composed of DNA obtained by modular exchange between different species of lactic acid bacteria. Based on sequence data and the derived presence of certain genes and proteins, the plasmid pGL2 appears to replicate via a rolling-circle mechanism, while the other four plasmids appear to belong to the group of lactococcal theta-type replicons. The plasmids pGL1, pGL2 and pGL5 encode putative proteins related with bacteriocin synthesis and bacteriocin secretion and immunity. The plasmid pGL5 harbors genes (txn, orf5 and orf25) encoding proteins that could be considered putative virulence factors. The gene txn encodes a protein with an enzymatic domain corresponding to the family actin-ADP-ribosyltransferases toxins, which are known to play a key role in pathogenesis of a variety of bacterial pathogens. The genes orf5 and orf25 encode two putative surface proteins containing the cell wall-sorting motif LPXTG, with mucin-binding and collagen-binding protein domains, respectively. These proteins could be involved in the adherence of L. garvieae to mucus from the intestine, facilitating further interaction with intestinal epithelial cells and to collagenous tissues such as the collagen-rich heart valves. To our knowledge, this is the first report on the characterization of plasmids in a human clinical strain of this pathogen




Aguado-Urda M., Gibello A., Blanco MM., Lopez-Campos GH., Cutuli MT. and Fernandez-Garayzabal JF..




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Characterization of plasmids in a human clinical strain of Lactococcus garvieae

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Characterization of plasmids in a human clinical strain of Lactococcus garvieae


Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIICentro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP). Instituto de Salud Carlos III (ISCIII).







PLoS ONE
FACTOR YEAR Q
3.730 2012

NLMID: 101285081

PMID: 22768237

ISSN: 1932-6203



TITLE: Characterization of plasmids in a human clinical strain of Lactococcus garvieae


JOURNAL: PLoS ONE


NUMERACIÓN: 7(6):e40119


AÑO: 2012


PUBLISHER: Public Library Science


AUTHORS: Aguado-Urda M., Gibello A., Blanco MM., Lopez-Campos GH., Cutuli MT. and Fernandez-Garayzabal JF..


VISAVET PARTICIPANTS


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/ 10.1371/journal.pone.0040119


CITE THIS PUBLICATION:

Aguado-Urda M., Gibello A., Blanco MM., Lopez-Campos GH., Cutuli MT. and Fernandez-Garayzabal JF. Characterization of plasmids in a human clinical strain of Lactococcus garvieae. PLoS ONE. 7(6):e40119. 2012. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0040119


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