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Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm

Veterinary Microbiology publish this investigation article

May 16th, 2022

Resistance to third- and fourth-generation cephalosporins in Escherichia coli is mainly due to extended-spectrum beta-lactamases (ESBL) and AmpC cephalosporinases, which have been increasingly reported, mainly in isolates from humans and poultry.
The aim of this study was to address the flow of antimicrobial resistance determinants in the full laying hen production cycle (four batches followed from day-old chicks to 83/84-week-old layers), using cephalosporin-resistant E. coli as a model and their characterization using whole genome sequencing (WGS).
Fifteen out of 22 samples analysed yielded growth on MacConkey agar with cefotaxime (1 mg/L). Of these, 141 isolates were identified as E. coli and 47 were characterized by WGS.
Genes detected were three ESBL (blaCTX-M-1 (n = 19); blaCTX-M-14 (n = 1); and blaSHV-12 (n = 9)) and one AmpC (blaCMY-2 (n = 13)). Some isolates only harboured blaTEM-1B (n = 2) or blaTEM-1D (n = 1).
IncI1 plasmids were the main platform for ESBL/AmpC genes. In addition, five clones were identified harbouring blaCTX-M-1 (two), blaSHV-12 (one) and blaCMY-2 (two), drawing a clone-plasmid mixed flow model.
Gene blaCTX-M-1 was found in the chromosomal DNA of clone 1 over 14 months, and in IncI1/ST3 plasmids over six months; over six months blaSHV-12 was found harboured by clone 3 (IncI1/ST26 plasmids), and 15 months later in a non-replicon detected plasmid. Finally, blaCMY-2 spread for at least 16 months, mainly by IncK2 (including clone 4) and IncI1/ST12 (clone 5) plasmids.
Proper use of antimicrobials should be combined with other farm management strategies for the effective control of cephalosporin-resistant E. coli isolates in commercial layer farms




Aldea-Ramos I., Gibello A., Hernandez M., Leekitcharoenphon P., Bortolaia V. and Moreno MA.




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Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm

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Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm



Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto Tecnológico Agrario de Castilla y LeónLaboratorio de Biología Molecular y Microbiología. Instituto Tecnológico Agrario de Castilla y León (ITACyL).

Technical University of DenmarkResearch Group for Genomic Epidemiology. National Food Institute (DTU Food). Technical University of Denmark (DTU).

Statens Serum InstitutDepartment of Bacteria. Parasites and Fungi. Statens Serum Institut (SSI).







Veterinary Microbiology
FACTOR YEAR Q
3.293 2020

NLMID: 7705469

PMID: 35640410

ISSN: 0378-1135



TITLE: Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 270:109453


AÑO: 2022


PUBLISHER: Elsevier Science Publishers


AUTHORS: Aldea-Ramos I., Gibello A., Hernandez M., Leekitcharoenphon P., Bortolaia V. and Moreno MA.


DOI: https://doi.org/10.1016/j.vetmic.2022.109453


CITE THIS PUBLICATION:

Aldea-Ramos I., Gibello A., Hernandez M., Leekitcharoenphon P., Bortolaia V. and Moreno MA. Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm. Veterinary Microbiology. 270:109453. 2022. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2022.109453


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