Poster communication in 9th Biennial Conference of the European Wildlife Disease Association

September 13^rd, 2010

Palomo G., Campos MJ., Ugarte-Ruiz M., Castilla C., Martinez R., Vadillo S., Piriz S. and Quesada A.

Salmonella colonizes the gut of a wide range of animals, from insects to mammals. Although non typhoid salmonellosis is the most common food borne disease in the North hemisphere, there are just a few studies focused on the importance of wildlife in its epidemiology. . We have analyzed 17 salmonella strains (4,5,12:i:-, Anatum, Bredeney, Choleraesuis, Enteritidis, Mikawasima and Typhimurium serovars) isolated from wild animals in the Veterinarian Hospitals of the Cáceres and Córdoba Universities (South west of Spain) , between 1998 and 2008. Fourteen different genotypes were evidenced by pulse field gel electrophoresis (PFGE) according to PulseNet protocols established by VISAVET laboratories (Madrid-Complutense University). Minimum inhibitory concentrations (MIC`s) for 23 antimicrobial agents were calculated by microdilution broth, according to the standard defined by the EFSA. Eleven strains resulted multidrug-resistant (according to EUCAST cut off values) whilst only four were totally sensitive to all tested antimicrobials. For the eight quinolone resistant strains, genes gyrA, gyrB, parC and parE were analyzed to detect the presence of mutations in their quinolone resistance determining regions (QRDR). Five strains share S83Y alleles of gyrA, whereas a single case of the allele S83F was found in a salmonella isolated from a white stork (Ciconia ciconia). In addition, the occurrence of class 1 integrons (int1) was revealed in the 47% of strains and correlated to the ACSSuT resistance profile. Thanks to amplification of gene-cassettes from conserved regions (CS), four genes associated to int1 were sequenced: aadA2 + bla-PSE1; aadA1 + dhfrVII; dhfrVII; and aadA1. These results support the high prevalence of antimicrobial resistance in Salmonella strains isolated from wildlife, where some of the determinants found are closely related to those found in antibiotic resistant salmonella from humans (gyrA-S83F and ciprofloxacin resistance, among others). Thus, wild fauna might play an important role for the spreading of antibiotic resistance

	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).