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Comparative genomics of quinolone-resistantand susceptibleCampylobacter jejuniof oultry origin from major poultry producing European countries (GENCAMP)

EFSA Journal publish this article

March 12nd, 2018

Leekitcharoenphon P., Garcia-Graels C., Botteldoorn N., Dierick K., Kempf I., Olkkola S., Rossi M., Nykasenoja S., Malorny B., Stingl K., Battisti A., Franco A., Mossong J., Veldman K., Mevius D., Wasyl D., Wieczorek K., Osek J., Clemente L., Lacatus A., Nicorescu I., Aguero Garcia M., de Frutos Escobar C., Duran Ferrer M., Ugarte-Ruiz M., Anjum MF., Teale C., Agren J., Hendriksen RS. and Aarestrup FM.


A total of 502 Campylobacter jejuniisolates from poultry in 12 different European countries(10 of them the largest poultry production countries in Europe)werewhole genome sequencedto examine the genomic diversity of fluoroquinolone resistant (FQ-R) and susceptible(FQ-S)C. jejuniacross the poultry producing European countries and to determine whether the emergence of fluoroquinolone resistance among C.jejuniis related to the transmission through countries or to the selection through fluoroquinolone use in the individual countries. A high genomic diversity was observed. The isolates clustered in fourmain clusters. All trees revealed that the isolates were clustered according to the presence/absence of the gyrA mutationscausing fluoroquinolone resistance and ST-types. The cgMLSTtrees of only FQ-R and FQ-S isolates showed that isolates from the same country of origin were distributed into multiple clusters similarly to the trees combining FQ-R and FQ-S isolates. The different phylogenetic methods,ranging from single nucleotide polymorphisms analysisto gene-by-gene approaches such as rMLST, cgMLST, wgMLST and core genome tree,provided concordant results, but it is not known which is the most accurate methodfor identifyingthe country of originof the isolates.Allele frequency analysis of isolatesunder this study and a selection of previously published C.jejunigenomes inENAshowed association of geographical origin of poultry C. jejunipopulations between Romania-Poland, Italy-Germany-England, Portugal-The Netherlands and USA-Luxemburg. Allele frequency and phylogenetic analysis indicated that the isolates from Finland were genetically different from C. jejunipopulations from other European countriesincluded in this study




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Comparative genomics of quinolone-resistantand susceptibleCampylobacter jejuniof oultry origin from major poultry producing European countries (GENCAMP)

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Participants:

Technical University of DenmarkResearch Group for Genomic Epidemiology. National Food Institute (DTU Food). Technical University of Denmark (DTU). 111

Institut Scientifique Santé Publique (WIV-ISP). 111

Universidad de ZaragozaFacultad de Veterinaria. Universidad de Zaragoza (UNIZAR). 111

111

Finnish Food Safety AuthorityMicrobiology Research Unit. Finnish Food Safety Authority (EVIRA). 111

Federal Institute for Risk AssessmentFederal Institute for Risk Assessment (BfR). 111

Istituto Zooprofilattico Sperimentale delle Regioni Lazio e ToscanaIstituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana (IZSLT). 111

Laboratoire National de Santé. 111

Wageningen UniversityCentral Veterinary Institute. Wageningen University (UR). 111

Department of Hygiene of Food of Animal Origin. National Veterinary Research Institute (PIWET). 111

National Reference Laboratory for Bovine Tuberculosis. 111

Instituto Nacional de Investigação Agrária e Veterinária (INIAV). 111

Institute for Hygiene and Veterinary Public Health (IHVPH). 111

Laboratorio Central de Veterinaria-Sanidad Animal. 111

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). 111

Animal and Plant Health AgencyDepartment of Bacteriology. Animal and Plant Health Agency (APHA). 111

National Veterinary InstituteDepartment of Microbiology. National Veterinary Institute (SVA). 111








TITLE: Comparative genomics of quinolone-resistantand susceptibleCampylobacter jejuniof oultry origin from major poultry producing European countries (GENCAMP)


TYPE: Article


AUTHORS: Leekitcharoenphon P., Garcia-Graels C., Botteldoorn N., Dierick K., Kempf I., Olkkola S., Rossi M., Nykasenoja S., Malorny B., Stingl K., Battisti A., Franco A., Mossong J., Veldman K., Mevius D., Wasyl D., Wieczorek K., Osek J., Clemente L., Lacatus A., Nicorescu I., Aguero Garcia M., de Frutos Escobar C., Duran Ferrer M., Ugarte-Ruiz M., Anjum MF., Teale C., Agren J., Hendriksen RS. and Aarestrup FM.


VISAVET PARTICIPANTS


María Ugarte Ruiz

JOURNAL: EFSA Journal


LANGUAJE: Spanish


DATE: March 12nd, 2018


PUBLISHING COMPANY: European Food Safety Authority (EFSA)


DOI: https://doi.org/ 10.2903/sp.efsa.2018.EN-1398


CITE THIS PUBLICATION:

Leekitcharoenphon P., Garcia-Graels C., Botteldoorn N., Dierick K., Kempf I., Olkkola S., Rossi M., Nykasenoja S., Malorny B., Stingl K., Battisti A., Franco A., Mossong J., Veldman K., Mevius D., Wasyl D., Wieczorek K., Osek J., Clemente L., Lacatus A., Nicorescu I., Aguero Garcia M., de Frutos Escobar C., Duran Ferrer M., Ugarte-Ruiz M., Anjum MF., Teale C., Agren J., Hendriksen RS. and Aarestrup FM. Comparative genomics of quinolone-resistantand susceptibleCampylobacter jejuniof oultry origin from major poultry producing European countries (GENCAMP).. EFSA Journal. European Food Safety Authority (EFSA). 2018. (Article)


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