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Comparison of two biochemical methods for identifying Corynebacterium pseudotuberculosis isolated from sheep and goats

Investigation published in The Veterinary Journal

June 1st, 2013

The biochemical pattern of Cowan and Steel (BPCS) was compared with a commercial biochemical strip for the identification of Corynebacterium pseudotuberculosis isolated from small ruminants. On 16S rRNA gene sequencing, 40/78 coryneform isolates from the lymph nodes of sheep and goats with lesions resembling caseous lymphadenitis were identified as C. pseudotuberculosis. The sensitivities of the BPCS and the commercial biochemical strip relative to 16S rRNA sequencing were 80% and 85%, and their specificities were 92.1% and 94.7%, respectively; the level of agreement between the BPCS and the commercial biochemical strip was high (κ=0.82). Likelihood ratios for positive and negative results were 10.0 and 0.22 for the BPCS, and 16.0 and 0.16 for the commercial biochemical strip, respectively. These results indicate that the BPCS and the commercial biochemical strip are both useful for identifying C. pseudotuberculosis in veterinary microbiology laboratories




Huerta B., Gomez-Gascon L., Vela AI., Fernandez-Garayzabal JF., Casamayor A., Tarradas C. and Maldonado A.




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Comparison of two biochemical methods for identifying Corynebacterium pseudotuberculosis isolated from sheep and goats

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Comparison of two biochemical methods for identifying Corynebacterium pseudotuberculosis isolated from sheep and goats



Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad de CórdobaDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







The Veterinary Journal
FACTOR YEAR Q
2.165 2013

NLMID: 9706281

PMID: 23352345

ISSN: 1090-0233



TITLE: Comparison of two biochemical methods for identifying Corynebacterium pseudotuberculosis isolated from sheep and goats


JOURNAL: Vet. J.


NUMERACIÓN: 196(3):552-4


AÑO: 2013


PUBLISHER: Elsevier SCI LTD


AUTHORS: Huerta B., Gomez-Gascon L., Vela AI., Fernandez-Garayzabal JF., Casamayor A., Tarradas C. and Maldonado A.


VISAVET PARTICIPANTS


3rd
Ana Isabel Vela Alonso
4th
José Francisco Fernández-Garayzabal Fernández
5th
Almudena Casamayor Sanz

DOI: https://doi.org/ 10.1016/j.tvjl.2012.12.008


CITE THIS PUBLICATION:

Huerta B., Gomez-Gascon L., Vela AI., Fernandez-Garayzabal JF., Casamayor A., Tarradas C. and Maldonado A. Comparison of two biochemical methods for identifying Corynebacterium pseudotuberculosis isolated from sheep and goats. The Veterinary Journal. 196(3):552-4. 2013. (A). ISSN: 1090-0233. DOI: 10.1016/j.tvjl.2012.12.008


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