Investigation article published in The Journal of Veterinary Medical Science

December 28^th, 2012

A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed high Stimulation Index (SI) compared to the positive control (LPS). In a following animal vaccination-challenge experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period

Guo X., Jia H., Yuan W., Zhang Q., Hou S., Sun Y., Zhu G., Zhu H. and Sanchez-Vizcaino JM..

Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo

Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo

	Servicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Institute of Animal Science (IAS). Chinese Academy of Agricultural Sciences (CAAS).
	College of Veterinary Medicine. Yangzhou University (YZU).
	China Animal Husbandry Group Research Institute (CAHG).
	Xinjiang Tiankang Animal Science Bio-Technology.