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Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo

Investigation article published in The Journal of Veterinary Medical Science

December 28th, 2012

A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed high Stimulation Index (SI) compared to the positive control (LPS). In a following animal vaccination-challenge experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period




Guo X., Jia H., Yuan W., Zhang Q., Hou S., Sun Y., Zhu G., Zhu H. and Sanchez-Vizcaino JM..




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Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo

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Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo


Participants:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Institute of Animal Science (IAS). Chinese Academy of Agricultural Sciences (CAAS).

Yangzhou UniversityCollege of Veterinary Medicine. Yangzhou University (YZU).

China Animal Husbandry Group Research Institute (CAHG).

Xinjiang Tiankang Animal Science Bio-Technology.







FACTOR YEAR Q
0.876 2012

NLMID: 9105360

PMID: 22814086

ISSN: 0916-7250



TITLE: Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo


JOURNAL: J. Vet. Med. Sci.


NUMERACIÓN: 74(12):1647-50


AÑO: 2012


PUBLISHER: Japanese Society Of Veterinary Science


AUTHORS: Guo X., Jia H., Yuan W., Zhang Q., Hou S., Sun Y., Zhu G., Zhu H. and Sanchez-Vizcaino JM..


VISAVET PARTICIPANTS


José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/ 10.1292/jvms.11-0543


CITE THIS PUBLICATION:

Guo X., Jia H., Yuan W., Zhang Q., Hou S., Sun Y., Zhu G., Zhu H. and Sanchez-Vizcaino JM. Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo. The Journal of Veterinary Medical Science . 74(12):1647-50. 2012. (A). ISSN: 0916-7250. DOI: 10.1292/jvms.11-0543


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