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Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA

Investigation published in PLoS ONE

September 9th, 2016

In the absence of effective vaccine(s), control of African swine fever caused by African swine fever virus (ASFV) must be based on early, efficient, cost-effective detection and strict control and elimination strategies. For this purpose, we developed an indirect ELISA capable of detecting ASFV antibodies in either serum or oral fluid specimens. The recombinant protein used in the ELISA was selected by comparing the early serum antibody response of ASFV-infected pigs (NHV-p68 isolate) to three major recombinant polypeptides (p30, p54, p72) using a multiplex fluorescent microbead-based immunoassay (FMIA). Non-hazardous (non-infectious) antibody-positive serum for use as plate positive controls and for the calculation of sample-to-positive (S:P) ratios was produced by inoculating pigs with a replicon particle (RP) vaccine expressing the ASFV p30 gene. The optimized ELISA detected anti-p30 antibodies in serum and/or oral fluid samples from pigs inoculated with ASFV under experimental conditions beginning 8 to 12 days post inoculation. Tests on serum (n = 200) and oral fluid (n = 200) field samples from an ASFV-free population demonstrated that the assay was highly diagnostically specific. The convenience and diagnostic utility of oral fluid sampling combined with the flexibility to test either serum or oral fluid on the same platform suggests that this assay will be highly useful under the conditions for which OIE recommends ASFV antibody surveillance, i.e., in ASFV-endemic areas and for the detection of infections with ASFV isolates of low virulence




Gimenez-Lirola LG., Mur L., Rivera B., Mogler M., Sun Y., Lizano S., Goodell C., Harris DL., Rowland DD., Gallardo C., Sanchez-Vizcaino JM. and Zimmermman J.




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Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA

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Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA


Participants:

Iowa State UniversityCollege of Veterinary Medicine. Iowa State University (ISU).

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Harrisvaccines, Inc.

Iowa State UniversityCollege of Liberal Arts and Sciences. Iowa State University (ISU).

Idexx LaboratoriesIdexx Laboratories (IDEXX).

Kansas State UniversityCollege of Veterinary Medicine. Kansas State University (KSU).

Instituto Nacional de Investigación y Tecnología Agraria y AlimentariaCentro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).







PLoS ONE
FACTOR YEAR Q
2.806 2016

NLMID: 101285081

PMID: 27611939

ISSN: 1932-6203



TITLE: Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA


JOURNAL: PLoS ONE


NUMERACIÓN: 11(9):e0161230


AÑO: 2016


PUBLISHER: Public Library Science


AUTHORS: Gimenez-Lirola LG., Mur L., Rivera B., Mogler M., Sun Y., Lizano S., Goodell C., Harris DL., Rowland DD., Gallardo C., Sanchez-Vizcaino JM. and Zimmermman J.


VISAVET PARTICIPANTS


3rd
Belén Rivera Arroyo
11th
José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/ 10.1371/journal.pone.0161230


CITE THIS PUBLICATION:

Gimenez-Lirola LG., Mur L., Rivera B., Mogler M., Sun Y., Lizano S., Goodell C., Harris DL., Rowland DD., Gallardo C., Sanchez-Vizcaino JM. and Zimmermman J. Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA. PLoS ONE. 11(9):e0161230. 2016. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0161230


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