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Determination of the immunotoxic potential of heavy metals on the functional activity of bottlenose dolphin leukocytes in vitro

Investigation article published in Veterinary Immunology and Immunopathology

February 15th, 2008

Heavy metals may affect the immune system of cetaceans. But no information exists on their effects on the bottlenose dolphin (Tursiops truncatus) immune system, although this species is a coastal top predator which can bioaccumulate high concentrations of them. This work studies the effects of Hg (1, 5 and 10mg/L), Al (2,5, 25 and 50mg/L), Cd (1, 10, 20 and 40mg/L), Pb (1, 10, 20 and 50mg/L) and Cr (1 and 10mg/L), on the function of phagocytes and lymphocytes isolated from the peripheral blood of bottlenose dolphins under in vitro conditions. Cell viability, apoptosis, lymphocyte proliferation and phagocytosis were evaluated. Viability and lymphoproliferation were measured with Alamar Blue assay, and apoptosis and phagocytosis were evaluated with flow cytometry. Apoptosis was detected as mechanism of cell death after cadmium and mercury exposure. A significant reduction in the lymphoproliferative response was registered by exposure to 1mg/L of mercury, 10mg/L of cadmium and 50mg/L of lead. Decreased phagocytosis was also observed at 5mg/L of mercury, 50mg/L of aluminium and 10mg/L of cadmium. Chromium did not present any effects on any immune assay at the concentrations tested. The concentrations of heavy metals that were found to affect the functional activity of bottlenose dolphin leukocytes are within the environmental ranges reported in the tissues of bottlenose dolphins. These results support the hypothesis that exposure to these contaminants, particularly mercury and cadmium could lead to a reduction in host resistance to disease in these animals




Camara S., Munoz MJ., Carballo M. and Sanchez-Vizcaino JM..




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Determination of the immunotoxic potential of heavy metals on the functional activity of bottlenose dolphin leukocytes in vitro

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Determination of the immunotoxic potential of heavy metals on the functional activity of bottlenose dolphin leukocytes in vitro



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Centro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Ministerio de Ciencia e Innovación.







Veterinary Immunology and Immunopathology
FACTOR YEAR Q
1.907 2008

NLMID: 8002006

PMID: 17997166

ISSN: 0165-2427



TITLE: Determination of the immunotoxic potential of heavy metals on the functional activity of bottlenose dolphin leukocytes in vitro


JOURNAL: Vet. Immunol. Immunopathol.


NUMERACIÓN: 121(3-4):189-98


AÑO: 2008


PUBLISHER: Elsevier Scientific


AUTHORS: Camara S., Munoz MJ., Carballo M. and Sanchez-Vizcaino JM..


VISAVET PARTICIPANTS


José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/10.1016/j.vetimm.2007.09.009


CITE THIS PUBLICATION:

Camara S., Munoz MJ., Carballo M. and Sanchez-Vizcaino JM. Determination of the immunotoxic potential of heavy metals on the functional activity of bottlenose dolphin leukocytes in vitro. Veterinary Immunology and Immunopathology. 121(3-4):189-98. 2008. (A). ISSN: 0165-2427. DOI: 10.1016/j.vetimm.2007.09.009


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