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Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African Horse Sickness and Equine Infectious Anemia viruses

Journal of Virological Methods publish this investigation article

September 9th, 2016

African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis




Costa S., Sastre P., Perez T., Tapia I., Barrandeguy M., Sanchez-Vizcaino JM., Sanchez-Matamoros A., Wigdorovitz A., Sanz A. and Rueda P.




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Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African Horse Sickness and Equine Infectious Anemia viruses

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Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African Horse Sickness and Equine Infectious Anemia viruses


Participants:

Inmunología y Genética Aplicada, S.A.Inmunología y Genética Aplicada, S.A. (INGENASA).

Instituto Nacional de Tecnología AgropecuariaInstituto de Virología. Centro de Investigación de Ciencias Veterinarias y Agronómicas (CICVYA). Instituto Nacional de Tecnología Agropecuaria (INTA).

Escuela de Veterinaria. Universidad del Salvador (USAL).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Journal of Virological Methods
FACTOR YEAR Q
1.693 2016

NLMID: 8005839

PMID: 27616197

ISSN: 0166-0934



TITLE: Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African Horse Sickness and Equine Infectious Anemia viruses


JOURNAL: J. Virol. Methods


NUMERACIÓN: 237:127-131


AÑO: 2016


PUBLISHER: Elsevier/North-Holland Biomedical Press


AUTHORS: Costa S., Sastre P., Perez T., Tapia I., Barrandeguy M., Sanchez-Vizcaino JM., Sanchez-Matamoros A., Wigdorovitz A., Sanz A. and Rueda P.


VISAVET PARTICIPANTS


José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/ 10.1016/j.jviromet.2016.09.009


CITE THIS PUBLICATION:

Costa S., Sastre P., Perez T., Tapia I., Barrandeguy M., Sanchez-Vizcaino JM., Sanchez-Matamoros A., Wigdorovitz A., Sanz A. and Rueda P. Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African Horse Sickness and Equine Infectious Anemia viruses. Journal of Virological Methods. 237:127-131. 2016. (A). ISSN: 0166-0934. DOI: 10.1016/j.jviromet.2016.09.009


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