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Development of a Suspension Microarray for the Genotyping of African Swine Fever Virus Targeting the SNPs in the C-Terminal End of the p72 Gene Region of the Genome

Oral communication in 6th EPIZONE Annual Meeting "Viruses on the move" 

June 12nd, 2012

Leblanc L., Cortey M., Fernandez-Pinero J., Gallardo C., Masembe C., Okurut AR., Heath L., Van Heerden J., Sanchez-Vizcaino JM., Stahl K. and Belak S.

African swine fever virus (ASFV) causes catastrophic disease in domestic pigs, with high rates of morbidity and mortality, which can reach 100%. To date, the serological diagnosis has the drawback of not being able to differentiate variants of this virus. Previous studies have identified 22 genotypes based on sequence variation in the C-terminal region of the p72 gene, which has become the standard for categorizing ASFVs. A genotyping assay has been developed using a segment of PCR-amplified genomic DNA of approximately 450 bp, which encompasses the C-terminal end of the p72 gene. Paired DNA probes which are identical except for a single nucleotide polymorphism (SNP) at the centre position, were designed to either individually or in combination differentiate between the 22 genotypes. The assay was developed using Luminex xMAP technology. Characterization of the sample was performed by comparing fluorescence of the paired SNP probes, i.e. the probe with higher
fluorescence in a pair identified the SNP that a particular sample possessed. In the final assay, a total of 52 probes were employed, 24 SNP pairs and 4 general detection. One or more samples from each of the 22 genotypes were tested. The assay was able to detect and distinguish all 22 genotypes. Thus, the novel assay provides a powerful tool for the complex and rapid diagnosis of African swine fever




Participants:

National Veterinary InstituteNational Veterinary Institute (SVA).

Instituto Nacional de Investigación y Tecnología Agraria y AlimentariaCentro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).

Makerere UniversityMakerere University (MAK).

Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Swedish University of Agricultural Sciences (SLU).


Link to 6th EPIZONE Annual Meeting "Viruses on the move" 





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6th EPIZONE Annual Meeting "Viruses on the move" 


Jun 12nd-14th, 2012
Brighton
United Kingdom

TITLE: Development of a Suspension Microarray for the Genotyping of African Swine Fever Virus Targeting the SNPs in the C-Terminal End of the p72 Gene Region of the Genome


TYPE: Oral communication


AUTHORS: Leblanc L., Cortey M., Fernandez-Pinero J., Gallardo C., Masembe C., Okurut AR., Heath L., Van Heerden J., Sanchez-Vizcaino JM., Stahl K. and Belak S.


VISAVET PARTICIPANTS


José Manuel Sánchez-Vizcaíno Rodríguez

DATE: June 12nd, 2012


CITE THIS COMMUNICATION:

Leblanc L., Cortey M., Fernandez-Pinero J., Gallardo C., Masembe C., Okurut AR., Heath L., Van Heerden J., Sanchez-Vizcaino JM., Stahl K. and Belak S. Development of a Suspension Microarray for the Genotyping of African Swine Fever Virus Targeting the SNPs in the C-Terminal End of the p72 Gene Region of the Genome. 6th EPIZONE Annual Meeting "Viruses on the move" , The Institute for Animal Health, Biotechnology and Biological Sciences Research Council, Visitbrighton, Brighton, United Kingdom, June 12nd, 2012. (Oral communication)


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