Poster communication in 2nd Congress of the European Association of Veterinary Laboratory Diagnosticians (EAVLD)

July 3^rd, 2012

Lopez L., Ranz A., Venteo A., Perez T., Ruiz T., de Juan L., Romero B., Boadella M., Gortazar C., Boniotti B., Pasquali P. and Rueda P.

Bovine tuberculosis (TB) is a chronic bacterial disease of animals and humans caused by M. bovis. In a large number of countries bovine tuberculosis is a major infectious disease among cattle, other domesticated animals, and certain wildlife populations. Transmission to humans constitutes a public health problem. For years eradication programmes have been carried out. Traditional mycobacterium culture remains the gold standard method for routine confirmation of infection but
Delayed Hypersensitivity test and gamma-interferon test are the ones used in these programmes. Detection of antibodies specific could be a useful alternative for TB diagnosis. Different antibodies detection assays have been described although all of
them present sensitivity problems The aim of this study has been to develop and validate multispecies assays based on Double Recognition ELISA (ELISA-DR) and Immunochromatography (IC) techniques for detection of antibodies specific of M. bovis. using the recombinant protein MPB83, expressed in insect cells

	Inmunología y Genética Aplicada, S.A. (INGENASA).
	Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).
	Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna (IZSLER).
	Istituto Superiore di Sanità.