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Equine infection with Leishmania spp. in Costa Rica: Study of five cases

Investigation published in Veterinary Medicine and Science

In Press

Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania spp. antigens in tissue sections. Laser-capture micro-dissection and quantitative real-time PCR techniques were carried out to detect the pathogen nucleic acid within the microscopic lesions. Histopathological analyses showed a granulomatous inflammation within the dermis, with multi-nucleated giant cells, macrophages, lymphocytes and few neutrophils and eosinophils. We detected the parasite by immunohistochemistry, using a rabbit polyclonal antibody raised against Leishmania spp. However, we could not identify Leishmania spp. by quantitative real-time PCR in formalin-fixed paraffin-embedded tissues, using specific primers for the conserved region in the minicircle of the Leishmania DNA kinetoplast. Our results emphasise the importance of Leishmania spp. not only as a causative agent of equine cutaneous disease in the New World, but also as a possible emerging pathogen. Leishmaniosis is one of the most prevalent parasitic public health problems worldwide, and equines may have a role in the epidemiology of the disease




Ortega-García MV., Salguero FJ., Garcia N., Dominguez M., Moreno I. and Berrocal A.




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Equine infection with Leishmania spp. in Costa Rica: Study of five cases

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Equine infection with Leishmania spp. in Costa Rica: Study of five cases



Participants:

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Ministerio de DefensaSubdirección de Sistemas Terrestres. Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

University of SurreyUniversity of Surrey.

Instituto de Salud Carlos IIICentro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

HistopatovetHistopatovet.



Related news in other media:

- Veterinarios españoles describen 5 casos de leishmaniosis equina - animalshealth.es







Veterinary Medicine and Science
FACTOR YEAR Q
1.950 2020

PMID: 34342158

ISSN: 2053-1095



TITLE: Equine infection with Leishmania spp. in Costa Rica: Study of five cases


JOURNAL: Vet. Med. Sci.


PUBLISHER: Wiley


AUTHORS: Ortega-García MV., Salguero FJ., Garcia N., Dominguez M., Moreno I. and Berrocal A.


VISAVET PARTICIPANTS


Nerea García Benzaquén

DOI: https://doi.org/ 10.1002/vms3.587


CITE THIS PUBLICATION:

Ortega-García MV., Salguero FJ., Garcia N., Dominguez M., Moreno I. and Berrocal A. Equine infection with Leishmania spp. in Costa Rica: Study of five cases. Veterinary Medicine and Science. In Press. (A). ISSN: 2053-1095. DOI: 10.1002/vms3.587


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