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Establishing Streptomycin Epidemiological Cut-Off Values for Salmonella and Escherichia coli

Investigation published in Microbial Drug Resistance (Larchmont, N.Y.)

February 1st, 2012

This study was conducted to elucidate the accuracy of the current streptomycin epidemiological cut-off value (ECOFF) for Escherichia coli and Salmonella spp. A total of 236 Salmonella enterica and 208 E. coli isolates exhibiting MICs between 4 and 32 mg/L were selected from 12 countries. Isolates were investigated by polymerase chain reaction for aadA, strA, and strB streptomycin resistance genes. Out of 236 Salmonella isolates, 32 (13.5%) yielded amplicons for aadA (n = 23), strA (n = 9), and strB (n = 11). None of the 60 Salmonella isolates exhibiting MIC 4 mg/L harbored resistance genes. Of the Salmonella isolates exhibiting MICs 8 mg/L, 16 mg/L, and 32 mg/L, 1.6%, 15%, and 39%, respectively, tested positive for one or more genes. For most monitoring programs, the streptomycin ECOFF for Salmonella is wild type (WT) ≤32 or ≤16 mg/L. A cut-off value of WT ≤32 mg/L would have misclassified 13.5% of the strains as belonging to the WT population, since this proportion of strains harbored resistance genes and exhibited MICs ≤32 mg/L. Out of 208 E. coli strains, 80 (38.5%) tested positive for aadA (n = 69), strA (n = 18), and strB (n = 31). Of the E. coli isolates exhibiting MICs of 4 mg/L, 8 mg/L, 16 mg/L, and 32 mg/L, 3.6%, 17.6%, 53%, and 82.3%, respectively, harbored any of the three genes. Based on the European Committee on Antimicrobial Susceptibility Testing guidelines (ECOFF ≤16 mg/L), 25% of the E. coli strains presenting MIC ≤16 mg/L would have been incorrectly categorized as belonging to the WT population. The authors recommend an ECOFF value of WT ≤16 mg/L for Salmonella and WT ≤8 mg/L for E. coli




Garcia-Migura L., Sunde M., Karlsmose S., Veldman K., Schroeter A., Guerra B., Granier SA., Perrin-Guyomard A., Gicquel-Bruneau M., Franco A., Englund S., Teale C., Heiska H., Clemente L., Boerlin P., Moreno MA., Daignault D., Mevius DJ., Hendriksen RS. and Aarestrup F.




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Establishing Streptomycin Epidemiological Cut-Off Values for Salmonella and Escherichia coli

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Establishing Streptomycin Epidemiological Cut-Off Values for Salmonella and Escherichia coli



Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Technical University of DenmarkNational Food Institute (DTU Food). Technical University of Denmark (DTU).

Seksjon for Bakteriologi. Veterinærinstituttet.

Wageningen UniversityCentral Veterinary Institute. Wageningen University (UR).

Federal Institute for Risk AssessmentFederal Institute for Risk Assessment (BfR).

Agence Nationale de Sécurité Sanitaire de l´alimentation, de l´environnement et du travailAgence Nationale de Sécurité Sanitaire de l´alimentation, de l´environnement et du travail (ANSES).

AFSSA—LERMVD.

Istituto Zooprofilattico Sperimentale delle Regioni Lazio e ToscanaIstituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana (IZSLT).

National Veterinary InstituteDepartment of Animal Health and Antimicrobial Strategies. National Veterinary Institute (SVA).

Department for Environment, Food and Rural AffairsVeterinary Laboratories Agency. Department for Environment, Food and Rural Affairs (Defra).

Finnish Food Safety AuthorityFinnish Food Safety Authority (EVIRA).

Laboratório Nacional de Investigação Veterinária (LNIV). Instituto Nacional de Recursos Biologicos (INRB).

Ontario Veterinary College. University of Guelph.

Public Health Agency of CanadaLaboratory for Foodborne Zoonoses. Public Health Agency of Canada (PHAC).







Microbial Drug Resistance (Larchmont, N.Y.)
FACTOR YEAR Q
2.364 2012

NLMID: 9508567

PMID: 21749212

ISSN: 1076-6294



TITLE: Establishing Streptomycin Epidemiological Cut-Off Values for Salmonella and Escherichia coli


JOURNAL: Microb. Drug Resist


NUMERACIÓN: 18(1):88-93


AÑO: 2012


PUBLISHER: Mary Ann Liebert, Inc.


AUTHORS: Garcia-Migura L., Sunde M., Karlsmose S., Veldman K., Schroeter A., Guerra B., Granier SA., Perrin-Guyomard A., Gicquel-Bruneau M., Franco A., Englund S., Teale C., Heiska H., Clemente L., Boerlin P., Moreno MA., Daignault D., Mevius DJ., Hendriksen RS. and Aarestrup F.


VISAVET PARTICIPANTS


Miguel Ángel Moreno Romo

DOI: https://doi.org/ 10.1089/mdr.2011.0064


CITE THIS PUBLICATION:

Garcia-Migura L., Sunde M., Karlsmose S., Veldman K., Schroeter A., Guerra B., Granier SA., Perrin-Guyomard A., Gicquel-Bruneau M., Franco A., Englund S., Teale C., Heiska H., Clemente L., Boerlin P., Moreno MA., Daignault D., Mevius DJ., Hendriksen RS. and Aarestrup F. Establishing Streptomycin Epidemiological Cut-Off Values for Salmonella and Escherichia coli. Microbial Drug Resistance (Larchmont, N.Y.). 18(1):88-93. 2012. (A). ISSN: 1076-6294. DOI: 10.1089/mdr.2011.0064


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