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Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs

Transboundary and Emerging Diseases publish this investigation article

December 1st, 2015

African swine fever (ASF) has persisted in Eastern Europe since 2007, and two endemic zones have been identified in the central and southern parts of the Russian Federation. Moderate- to low-virulent ASF virus isolates are known to circulate in endemic ASF-affected regions. To improve our knowledge of virus transmission in animals recovered from ASF virus infection, an experimental in vivo study was carried out. Four domestic pigs were inoculated with the NH/P68 ASF virus, previously characterized to develop a chronic form of ASF. Two additional in-contact pigs were introduced at 72 days post-inoculation (dpi) in the same box for virus exposure. The inoculated pigs developed a mild form of the disease, and the virus was isolated from tissues in the inoculated pigs up to 99 dpi (pigs were euthanized at 36, 65, 99 and 134 dpi). In-contact pigs showed mild or no clinical signs, but did become seropositive, and a transient viraemia was detected at 28 days post-exposure (dpe), thereby confirming late virus transmission from the inoculated pigs. Virus transmission to in-contact pigs occurred at four weeks post-exposure, over three months after the primary infection. These results highlight the potential role of survivor pigs in disease maintenance and dissemination in areas where moderate- to low-virulent viruses may be circulating undetected. This study will help design better and more effective control programmes to fight against this disease




Gallardo C., Soler A., Nieto R., Sanchez MA., Martins C., Pelayo V., Carrascosa AL., Revilla Y., Simon A., Briones V., Sanchez-Vizcaino JM. and Arias M.




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Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs

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Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs


Participants:

Instituto Nacional de Investigación y Tecnología Agraria y AlimentariaEuropean Union Reference Laboratory for African Swine Fever. Centro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).

Faculdade de Medicina Veterinária. Universidade Técnica de Lisboa (UTL).

Consejo Superior de Investigaciones CientíficasConsejo Superior de Investigaciones Científicas (CSIC).

Centro de Biología Molecular Severo Ochoa (CBMSO). Consejo Superior de Investigaciones Científicas (CSIC). Universidad Autónoma de Madrid (UAM).

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Transboundary and Emerging Diseases
FACTOR YEAR Q
2.714 2015

NLMID: 101319538

PMID: 26432068

ISSN: 1865-1674



TITLE: Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs


JOURNAL: Transbound. Emerg. Dis.


NUMERACIÓN: 62(6):612-22


AÑO: 2015


PUBLISHER: Blackwell Verlag


AUTHORS: Gallardo C., Soler A., Nieto R., Sanchez MA., Martins C., Pelayo V., Carrascosa AL., Revilla Y., Simon A., Briones V., Sanchez-Vizcaino JM. and Arias M.


VISAVET PARTICIPANTS


10
Víctor Briones Dieste
11th
José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/ 10.1111/tbed.12431


CITE THIS PUBLICATION:

Gallardo C., Soler A., Nieto R., Sanchez MA., Martins C., Pelayo V., Carrascosa AL., Revilla Y., Simon A., Briones V., Sanchez-Vizcaino JM. and Arias M. Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs. Transboundary and Emerging Diseases. 62(6):612-22. 2015. (A). ISSN: 1865-1674. DOI: 10.1111/tbed.12431


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