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Expression of immunoregulatory genes in peripheral blood mononuclear cells of European wild boar immunized with BCG

Investigation article published in Veterinary Microbiology

March 1st, 2009

The objective of this study was to analyze the expression of immunoregulatory genes in European wild boar (Sus scrofa) immunized with BCG. Eighteen immunoregulatory genes were selected for expression analysis based on their role in host immune response during tuberculosis and/or for their association with resistance to bovine tuberculosis in European wild boar populations. Initially, mRNA levels were analyzed by quantitative real-time reverse transcription PCR (qRT-PCR) in spleen samples from Mycobacterium bovis-infected (N=18) and uninfected (N=22) European wild boar. Statistical analysis of qRT-PCR data revealed that four genes, complement component C3, IFN-gamma, IL-4 and RANTES were downregulated in infected animals (P<0.05). These genes were selected for analysis of mRNA levels in peripheral blood mononuclear cells (PBMCs) from seven wild boar experimentally immunized with BCG and seven non-immunized controls. Blood was collected at 0, 5, 13 and 25 weeks post-immunization (wpi). The mRNA levels of IFN-gamma and C3 showed a peak (>15-fold increase) at 5 wpi, whereas transcripts for RANTES and IL-4 showed a peak (>2-fold increase) at 13 wpi in BCG-immunized animals when compared to non-immunized controls. The pattern of expression of these genes over the time provides the first description of BCG specific immune response in European wild boar. These results provide new insights into the molecular basis of wild boar response to M. bovis infection and BCG vaccination and may be used to monitor BCG vaccination in this species




Lastra JM., Galindo RC., Gortazar C., Ruiz-Fons F., Aranaz A. and de la Fuente J.




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Expression of immunoregulatory genes in peripheral blood mononuclear cells of European wild boar immunized with BCG

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Expression of immunoregulatory genes in peripheral blood mononuclear cells of European wild boar immunized with BCG



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Oklahoma State UniversityDepartament of Veterinary Pathobiology. Center for Veterinary Health Sciences (CVHS). Oklahoma State University (OSU).







Veterinary Microbiology
FACTOR YEAR Q
2.874 2009

NLMID: 7705469

PMID: 19095381

ISSN: 0378-1135



TITLE: Expression of immunoregulatory genes in peripheral blood mononuclear cells of European wild boar immunized with BCG


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 134(3-4):334-9


AÑO: 2009


PUBLISHER: Elsevier Science Publishers


AUTHORS: Lastra JM., Galindo RC., Gortazar C., Ruiz-Fons F., Aranaz A. and de la Fuente J.


DOI: https://doi.org/10.1016/j.vetmic.2008.08.026


CITE THIS PUBLICATION:

Lastra JM., Galindo RC., Gortazar C., Ruiz-Fons F., Aranaz A. and de la Fuente J. Expression of immunoregulatory genes in peripheral blood mononuclear cells of European wild boar immunized with BCG. Veterinary Microbiology. 134(3-4):334-9. 2009. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2008.08.026


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