Home \ Research \ Scientific publications \


Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages

The Journal of Antimicrobial Chemotherapy publish this investigation article

November 1st, 2020

Objectives: To investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles.
Methods: Several databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a marker in eight different plasmids, from different incompatibility groups, and with differing sizes and plasmid copy numbers. All plasmids were transformed into Escherichia coli bearing one of four different lysogenic phages. Upon induction, encapsidation of armA in phage particles was evaluated using qRT-PCR and Southern blotting.
Results: Multicopy plasmids carry a vast set of emerging clinically important antimicrobial resistance genes. However, 60% of these plasmids do not bear mobility (MOB) genes. When carried on these multicopy plasmids, mobilization of a marker gene armA into phage capsids was up to 10 000 times more frequent than when it was encoded by a large plasmid with a low copy number.
Conclusions: Multicopy plasmids and phages, two major mobile genetic elements (MGE) in bacteria, represent a novel high-efficiency transmission route of antimicrobial resistance genes that deserves further investigation




Rodriguez-Campoo R., Serna Bernaldo C., Ares M., Matamoros BR., Delgado-Blas JF., Montero N., Bernabe-Balas C., Wedel EF., Mendez IS., Muniesa M. and Gonzalez-Zorn B..




See this article
Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages

See it on NLM PubMed
Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages


Participants:

Universitat de BarcelonaDepartamento de Microbiología. Facultad de Biología. Universitat de Barcelona (UB).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







The Journal of Antimicrobial Chemotherapy
FACTOR YEAR Q
5.439 2019

NLMID: 7513617

PMID: 32719862

ISSN: 0305-7453



TITLE: Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages


JOURNAL: J. Antimicrob. Chemother.


NUMERACIÓN: 75(11):3173-3180


AÑO: 2020


PUBLISHER: Oxford University Press


AUTHORS: Rodriguez-Campoo R., Serna Bernaldo C., Ares M., Matamoros BR., Delgado-Blas JF., Montero N., Bernabe-Balas C., Wedel EF., Mendez IS., Muniesa M. and Gonzalez-Zorn B..


VISAVET PARTICIPANTS


3rd
Manuel Ares Arroyo
5th
José Francisco Delgado Blas
Last
Bruno González Zorn

DOI: https://doi.org/ 10.1093/jac/dkaa311


CITE THIS PUBLICATION:

Rodriguez-Campoo R., Serna Bernaldo C., Ares M., Matamoros BR., Delgado-Blas JF., Montero N., Bernabe-Balas C., Wedel EF., Mendez IS., Muniesa M. and Gonzalez-Zorn B. Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages. The Journal of Antimicrobial Chemotherapy. 75(11):3173-3180. 2020. (A). ISSN: 0305-7453. DOI: 10.1093/jac/dkaa311


UNITS: