Home \ Research \ Scientific publications \


First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis

Journal of Equine Veterinary Science publish this investigation article

June 1st, 2017

Strangles is one of the most frequently diagnosed equine respiratory infectious diseases in the world. It is caused by Streptococcus equi subsp. equi (S. equi), and it is an acute infection characterized by pyrexia, nasal discharge, pharyngitis, and abscessation of lymph nodes. Frequently, healthy horses might continue to harbor S. equi after clinical recovery. Although the genetic distance between S. equi isolates is short, strains can be differentiated by pulsed-field gel electrophoresis (PFGE) and single locus sequence typing for epidemiological studies. The aim of this study was to characterize by PFGE Argentine isolates of S. equi obtained from horses with acute strangles and those that had recovered. Bacterial isolation and identification of 80 S. equi isolates by phenotypic and genotypic tests were performed using samples from 29 horses with acute strangles and 95 from healthy animals. Also, the isolates were characterized by PFGE using Bsp120I and SmaI. Visual comparison of macrorestriction patterns generated with both enzymes revealed three different DNA fragment profiles with variations of one or two bands. Interestingly, an identical profile was found in isolates from the same horse and from horses that were infected at the same time, and the horses recovered from strangles continue to carry the same strain. Some vaccinated horses have been mild infected for a different strain from that of carriers suggesting other source of infection. This is the first molecular characterization of Argentine isolates of S. equi, which shows the presence of three strains between 2010 and 2013 in Buenos Aires




Bustos CP., Guida N., Casamayor A., Munoz AJ., Fernandez-Garayzabal JF. and Vela AI..




See this article
First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis




Participants:

Universidad de Buenos AiresCátedra de Enfermedades Infecciosas. Facultad de Ciencias Veterinarias. Universidad de Buenos Aires (UBA).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Journal of Equine Veterinary Science
FACTOR YEAR Q
0.880 2017

NLMID: 8216840

ISSN: 0737-0806



TITLE: First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis


JOURNAL: J. Equine Vet. Sci.


NUMERACIÓN: 53(2017):30-37


AÑO: 2017


PUBLISHER: Wildomar, CA : William E. Jones, c1981-


AUTHORS: Bustos CP., Guida N., Casamayor A., Munoz AJ., Fernandez-Garayzabal JF. and Vela AI..


VISAVET PARTICIPANTS


3rd
Almudena Casamayor Sanz
5th
José Francisco Fernández-Garayzabal Fernández
Last
Ana Isabel Vela Alonso

DOI: https://doi.org/ 10.1016/j.jevs.2017.01.007


CITE THIS PUBLICATION:

Bustos CP., Guida N., Casamayor A., Munoz AJ., Fernandez-Garayzabal JF. and Vela AI. First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis. Journal of Equine Veterinary Science. 53(2017):30-37. 2017. (A). ISSN: 0737-0806. DOI: 10.1016/j.jevs.2017.01.007


UNITS: