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Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains

Applied and Environmental Microbiology publish this investigation article

July 1st, 2013

Lactococcus garvieae 21881, isolated from a human clinical case, produces a novel class IId bacteriocin, garvicin A (GarA), which is specifically active against other L. garvieae strains, including fish and bovine pathogenic isolates. Purification from active supernatants, sequence analyses, and plasmid-curing experiments identified pGL5, one of the five plasmids found in L. garvieae 21881 (Aguado-Urda M, Gibello A, Blanco MM, López-Campos GH, Cutuli MT, Fernández-Garayzábal JF. 2012. PLoS ONE 7 (6):e40119. doi:10.1371/journal.pone.0040119), as the coding plasmid for the structural gene of GarA (lgnA), its putative immunity protein (lgnI), and the ABC-transporter and its accessory protein (lgnC and lgnD). Interestingly, pGL5-cured strains were still resistant to GarA. Other putative bacteriocins encoded by the remaining plasmids were not detected during purification pointing to GarA as the main inhibitor secreted by L. garvieae 21881. Mode of action studies revealed a potent bactericidal activity of GarA. Moreover, transmission microscopy showed that GarA seems to act by inhibiting septum formation in L. garvieae cells. The potent and species-specific inhibition by GarA anticipates promising applications for the prevention or treatment of infections caused by pathogenic strains of L. garvieae in both veterinary and clinical settings




Maldonado-Barragan A., Cardenas N., Martinez-Fernandez B., Ruiz-Barba JL., Fernandez-Garayzabal JF., Rodriguez JM. and Gibello A.




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Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains

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Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains


Participants:

Consejo Superior de Investigaciones CientíficasInstituto de la Grasa. Consejo Superior de Investigaciones Científicas (CSIC).

Universidad ComplutenseDepartamento de Nutrición, Bromatología y Tecnología de los Alimentos. Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Consejo Superior de Investigaciones CientíficasInstituto de Productos Lácteos de Asturias (IPLA). Consejo Superior de Investigaciones Científicas (CSIC).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Applied and Environmental Microbiology
FACTOR YEAR Q
3.952 2013

NLMID: 7605801

PMID: 23666326

ISSN: 0099-2240



TITLE: Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains


JOURNAL: Appl. Environ. Microbiol.


NUMERACIÓN: 79(14):4336-46


AÑO: 2013


PUBLISHER: American Society for Microbiology


AUTHORS: Maldonado-Barragan A., Cardenas N., Martinez-Fernandez B., Ruiz-Barba JL., Fernandez-Garayzabal JF., Rodriguez JM. and Gibello A.


VISAVET PARTICIPANTS


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/ 10.1128/AEM.00830-13


CITE THIS PUBLICATION:

Maldonado-Barragan A., Cardenas N., Martinez-Fernandez B., Ruiz-Barba JL., Fernandez-Garayzabal JF., Rodriguez JM. and Gibello A. Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains. Applied and Environmental Microbiology. 79(14):4336-46. 2013. (A). ISSN: 0099-2240. DOI: 10.1128/AEM.00830-13


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