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Genomic characterization of multidrug-resistant Salmonella serovar Kentucky ST198 isolated in poultry flocks in Spain (2011-2017)

Investigation article published in Microbial Genomics

March 1st, 2022

Salmonella Kentucky is commonly found in poultry and rarely associated with human disease. However, a multidrug-resistant (MDR) S. Kentucky clone [sequence type (ST)198] has been increasingly reported globally in humans and animals. Our aim here was to assess if the recently reported increase of S. Kentucky in poultry in Spain was associated with the ST198 clone and to characterize this MDR clone and its distribution in Spain. Sixty-six isolates retrieved from turkey, laying hen and broiler in 2011-2017 were subjected to whole-genome sequencing to assess their sequence type, genetic relatedness, and presence of antimicrobial resistance genes (ARGs), plasmid replicons and virulence factors. Thirteen strains were further analysed using long-read sequencing technologies to characterize the genetic background associated with ARGs. All isolates belonged to the ST198 clone and were grouped in three clades associated with the presence of a specific point mutation in the gyrA gene, their geographical origin and isolation year. All strains carried between one and 16 ARGs whose presence correlated with the resistance phenotype to between two and eight antimicrobials. The ARGs were located in the Salmonella genomic island (SGI-1) and in some cases (bla SHV-12 , catA1, cmlA1, dfrA and multiple aminoglycoside-resistance genes) in IncHI2/IncI1 plasmids, some of which were consistently detected in different years/farms in certain regions, suggesting they could persist over time. Our results indicate that the MDR S. Kentucky ST198 is present in all investigated poultry hosts in Spain, and that certain strains also carry additional plasmid-mediated ARGs, thus increasing its potential public health significance




Samper-Cativiela C., Bernabé Diéguez B., Trigo de Roza F., Ugarte-Ruiz M., Elnekave E., Lim S., Hernandez M., Abad D., Collado S., Saez-Llorente JL., De Frutos C., Aguero M., Moreno MA., Escudero JA. and Alvarez J..




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Genomic characterization of multidrug-resistant Salmonella serovar Kentucky ST198 isolated in poultry flocks in Spain (2011-2017)

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Genomic characterization of multidrug-resistant Salmonella serovar Kentucky ST198 isolated in poultry flocks in Spain (2011-2017)



Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Tecnologías y Servicios Agrarios S.A. TRAGSATEC.

Hebrew University of JerusalemThe Robert H. Smith Faculty of Agriculture. Food and Environment Sciences. Hebrew University of Jerusalem.

University of MinnesotaDepartment of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).

University of MinnesotaBioinformatics and Computational Biology Program. University of Minnesota (UMM).

Instituto Tecnológico Agrario de Castilla y LeónInstituto Tecnológico Agrario de Castilla y León (ITACyL).

Ministerio de Agricultura, Pesca y AlimentaciónSubdirección General de Sanidad e Higiene Animal y Trazabilidad. Dirección General de la Producción Agraria. Ministerio de Agricultura, Pesca y Alimentación (MAPA).

Ministerio de Agricultura, Pesca y AlimentaciónLaboratorio Central de Veterinaria (LCV). Ministerio de Agricultura, Pesca y Alimentación (MAPA).







Microbial Genomics
FACTOR YEAR Q
5.237 2020

PMID: 35259085

ISSN: 257-5858



TITLE: Genomic characterization of multidrug-resistant Salmonella serovar Kentucky ST198 isolated in poultry flocks in Spain (2011-2017)


JOURNAL: Microb. Genomics


NUMERACIÓN: 8(3)


AÑO: 2022


PUBLISHER: Microbiology Society


AUTHORS: Samper-Cativiela C., Bernabé Diéguez B., Trigo de Roza F., Ugarte-Ruiz M., Elnekave E., Lim S., Hernandez M., Abad D., Collado S., Saez-Llorente JL., De Frutos C., Aguero M., Moreno MA., Escudero JA. and Alvarez J..


VISAVET PARTICIPANTS


First
Clara Samper Cativiela
4th
María Ugarte Ruiz
14th
José Antonio Escudero García-Calderón
Last
Julio Álvarez Sánchez

DOI: https://doi.org/10.1099/mgen.0.000773


CITE THIS PUBLICATION:

Samper-Cativiela C., Bernabé Diéguez B., Trigo de Roza F., Ugarte-Ruiz M., Elnekave E., Lim S., Hernandez M., Abad D., Collado S., Saez-Llorente JL., De Frutos C., Aguero M., Moreno MA., Escudero JA. and Alvarez J. Genomic characterization of multidrug-resistant Salmonella serovar Kentucky ST198 isolated in poultry flocks in Spain (2011-2017). Microbial Genomics. 8(3). 2022. (A). ISSN: 257-5858. DOI: 10.1099/mgen.0.000773


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