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Genomic comparison between Mycobacterium bovis and Mycobacterium microti and in silico analysis of peptide-based biomarkers for serodiagnosis

Investigation article published in Frontiers in veterinary science

September 20th, 2024

In recent years, there has been an increase in the number of reported cases of Mycobacterium microti infection in various animals, which can interfere with the ante-mortem diagnosis of animal tuberculosis caused by Mycobacterium bovis. In this study, whole genome sequencing (WGS) was used to search for protein-coding genes to distinguish M. microti from M. bovis. In addition, the population structure of the available M. microti genomic WGS datasets is described, including three novel Belgian isolates from infections in alpacas. Candidate genes were identified by examining the presence of the regions of difference and by a pan-genome analysis of the available WGS data. A total of 80 genes showed presence-absence variation between the two species, including genes encoding Proline-Glutamate (PE), Proline-Proline-Glutamate (PPE), and Polymorphic GC-Rich Sequence (PE-PGRS) proteins involved in virulence and host interaction. Filtering based on predicted subcellular localization, sequence homology and predicted antigenicity resulted in 28 proteins out of 80 that were predicted to be potential antigens. As synthetic peptides are less costly and variable than recombinant proteins, an in silico approach was performed to identify linear and discontinuous B-cell epitopes in the selected proteins. From the 28 proteins, 157 B-cell epitope-based peptides were identified that discriminated between M. bovis and M. microti species. Although confirmation by in vitro testing is still required, these candidate synthetic peptides containing B-cell epitopes could potentially be used in serological tests to differentiate cases of M. bovis from M. microti infection, thus reducing misdiagnosis in animal tuberculosis surveillance




Moens C., Bogaerts B., Lorente-Leal V., Vanneste K., Keersmaeecker SCJ., Roosens NHC., Mostin L., Fretin D. and Marche S.




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Genomic comparison between Mycobacterium bovis and Mycobacterium microti and in silico analysis of peptide-based biomarkers for serodiagnosis

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Genomic comparison between Mycobacterium bovis and Mycobacterium microti and in silico analysis of peptide-based biomarkers for serodiagnosis



Participants:

Laboratory of Veterinary Bacteriology. Department of Animal Infectious Diseases. Sciensano.

Laboratory of Biochemistry and Genetics of Microorganisms. Louvain Institute of Biomolecular Science and Technology. Université Catholique de Louvain.

Transversal Activities in Applied Genomics. Sciensano.

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Experimental Center Machelen. Sciensano.







Frontiers in veterinary science
FACTOR YEAR Q
2.600 2023

NLMID: 101666658

PMID: 39372902

ISSN: 2297-1769



TITLE: Genomic comparison between Mycobacterium bovis and Mycobacterium microti and in silico analysis of peptide-based biomarkers for serodiagnosis


JOURNAL: Front Vet Sci


NUMERACIÓN: 11:1446930


AÑO: 2024


PUBLISHER: Lausanne : Frontiers Media S.A


AUTHORS: Moens C., Bogaerts B., Lorente-Leal V., Vanneste K., Keersmaeecker SCJ., Roosens NHC., Mostin L., Fretin D. and Marche S.


Víctor Lorente Leal

DOI: https://doi.org/10.3389/fvets.2024.1446930


CITE THIS PUBLICATION:

Moens C., Bogaerts B., Lorente-Leal V., Vanneste K., Keersmaeecker SCJ., Roosens NHC., Mostin L., Fretin D. and Marche S. Genomic comparison between Mycobacterium bovis and Mycobacterium microti and in silico analysis of peptide-based biomarkers for serodiagnosis. Frontiers in veterinary science. 11:1446930. 2024. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2024.1446930


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