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Global transcriptome analysis of Lactococcus garvieae strains in response to temperature

Investigation article published in PLoS ONE

October 4th, 2013

Lactococcus garvieae is an important fish and an opportunistic human pathogen. The genomic sequences of several L. garvieae strains have been recently published, opening the possibility of global studies on the biology of this pathogen. In this study, a whole genome DNA microarray of two strains of L. garvieae was designed and validated. This DNA microarray was used to investigate the effects of growth temperature (18°C and 37°C) on the transcriptome of two clinical strains of L. garvieae that were isolated from fish (Lg8831) and from a human case of septicemia (Lg21881). The transcriptome profiles evidenced a strain-specific response to temperature, which was more evident at 18°C. Among the most significant findings, Lg8831 was found to up-regulate at 18°C several genes encoding different cold-shock and cold-induced proteins involved in an efficient adaptive response of this strain to low-temperature conditions. Another relevant result was the description, for the first time, of respiratory metabolism in L. garvieae, whose gene expression regulation was temperature-dependent in Lg21881. This study provides new insights about how environmental factors such as temperature can affect L. garvieae gene expression. These data could improve our understanding of the regulatory networks and adaptive biology of this important pathogen




Aguado-Urda M., Gibello A., Blanco MM., Fernandez-Garayzabal JF., Lopez-Alonso V. and Lopez-Campos GH.




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Global transcriptome analysis of Lactococcus garvieae strains in response to temperature

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Global transcriptome analysis of Lactococcus garvieae strains in response to temperature


Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIInstituto de Salud Carlos III (ISCIII).

University of MelbourneMedical School. University of Melbourne.







PLoS ONE
FACTOR YEAR Q
3.534 2013

NLMID: 101285081

PMID: 24223997

ISSN: 1932-6203



TITLE: Global transcriptome analysis of Lactococcus garvieae strains in response to temperature


JOURNAL: PLoS ONE


NUMERACIÓN: 8(11):e79692


AÑO: 2013


PUBLISHER: Public Library Science


AUTHORS: Aguado-Urda M., Gibello A., Blanco MM., Fernandez-Garayzabal JF., Lopez-Alonso V. and Lopez-Campos GH.


VISAVET PARTICIPANTS


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/ 10.1371/journal.pone.0079692


CITE THIS PUBLICATION:

Aguado-Urda M., Gibello A., Blanco MM., Fernandez-Garayzabal JF., Lopez-Alonso V. and Lopez-Campos GH. Global transcriptome analysis of Lactococcus garvieae strains in response to temperature. PLoS ONE. 8(11):e79692. 2013. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0079692


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