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Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates

Investigation published in Veterinary Microbiology

August 15th, 2016

Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has emerged in animal production worldwide. Most LA-MRSA in Europe belong to the clonal complex (CC) 398. The reason for the LA-MRSA emergence is not fully understood. Besides antimicrobial agents used for therapy, other substances with antimicrobial activity applied in animal feed, including metal-containing compounds might contribute to their selection. Some of these genes have been found in various novel SCCmec cassettes. The aim of this study was to assess the occurrence of metal-resistance genes among a LA-S. aureus collection [n=554, including 542 MRSA and 12 methicillin-susceptible S. aureus (MSSA)] isolated from livestock and food thereof. Most LA-MRSA isolates (76%) carried at least one metal-resistance gene. Among the LA-MRSA CC398 isolates (n=456), 4.8%, 0.2%, 24.3% and 71.5% were positive for arsA (arsenic compounds), cadD (cadmium), copB (copper) and czrC (zinc/cadmium) resistance genes, respectively. In contrast, among the LA-MRSA non-CC398 isolates (n=86), 1.2%, 18.6% and 16.3% were positive for the cadD, copB and czrC genes, respectively, and none were positive for arsA. Of the LA-MRSA CC398 isolates, 72% carried one metal-resistance gene, and the remaining harboured two or more in different combinations. Differences between LA-MRSA CC398 and non-CC398 were statistically significant for arsA and czrC. The czrC gene was almost exclusively found (98%) in the presence of SCCmec V in both CC398 and non-CC398 LA-MRSA isolates from different sources. Regarding the LA-MSSA isolates (n=12), some (n=4) were also positive for metal-resistance genes. This study shows that genes potentially conferring metal-resistance are frequently present in LA-MRSA




Argudin MA., Lauzat B., Kraushaar B., Alba P., Agerso Y., Cavaco L., Butaye P., Porrero MC., Battisti A., Tenhagen BA., Fetsch A. and Guerra B.




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Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates

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Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates



Participants:

Federal Institute for Risk AssessmentDepartment of Biological Safety. Federal Institute for Risk Assessment (BfR).

Centrum voor Onderzoek in Diergeneeskunde en Agrochemie- Centre d´Etude et de Recherches Vétérinaires et AgrochimiquesCentrum voor Onderzoek in Diergeneeskunde en Agrochemie- Centre d´Etude et de Recherches Vétérinaires et Agrochimiques (CODA-CERVA).

Istituto Zooprofilattico Sperimentale delle Regioni Lazio e ToscanaIstituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana (IZSLT).

Technical University of DenmarkNational Food Institute (DTU Food). Technical University of Denmark (DTU).

Ross UniversityDepartment of Biosciences. School of Veterinary Medicine. Ross University.

University of GhentDepartment of Pathology, Bacteriology, and Poultry Diseases. Faculty of Veterinary Medicine. University of Ghent (UGENT).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
2.628 2016

NLMID: 7705469

PMID: 27374912

ISSN: 0378-1135



TITLE: Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 191:88-95


AÑO: 2016


PUBLISHER: Elsevier Science Publishers


AUTHORS: Argudin MA., Lauzat B., Kraushaar B., Alba P., Agerso Y., Cavaco L., Butaye P., Porrero MC., Battisti A., Tenhagen BA., Fetsch A. and Guerra B.


DOI: https://doi.org/10.1016/j.vetmic.2016.06.004


CITE THIS PUBLICATION:

Argudin MA., Lauzat B., Kraushaar B., Alba P., Agerso Y., Cavaco L., Butaye P., Porrero MC., Battisti A., Tenhagen BA., Fetsch A. and Guerra B. Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates. Veterinary Microbiology. 191:88-95. 2016. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2016.06.004


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