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Identification of putative volatile biomarkers of canine leishmaniasis in dog`s breath and hair employing a novel algorithm for automated chromatographic peak detection and matching

Investigation published in Analytical and Bioanalytical Chemistry

In Press

The analysis of the volatile compounds released by biological samples represents a promising approach for the non-invasive diagnosis of a disease. The present study, focused on a population of dogs infected with canine leishmaniasis, aimed to decipher the volatolomic profile associated with this disease in dogs, which represent the main animal reservoir for Leishmania pathogen transmission to humans. The volatiles emitted by the breath and hair of dogs were analysed employing the gas chromatography-mass spectrometry (GC-MS) technique. The acquired chromatograms were investigated using a novel algorithm developed in this study for automated chromatographic peak detection and matching in untargeted GC-MS analysis, which includes various steps that comprise noise reduction, m/z filtering, background subtraction, peak detection, peak matching, and generation of a peak table for compounds identification. The results revealed one tentative breath volatile biomarker and five tentative hair volatile biomarkers for the cutaneous form of the disease, which is characterised by skin ulcerations. Additionally, nine tentative breath volatile biomarkers and four tentative hair volatile biomarkers were found for the visceral form of the disease, which affects internal organs such as spleen, liver and bone marrow. All tentative biomarkers identified in this study were upregulated in cutaneous leishmaniasis, while in visceral leishmaniasis, all tentative biomarkers were upregulated in the breath and only one out of four in the hair. Only one compound (glyceryl monooleate) was identified as tentative volatile biomarker for both forms of the disease, in the hair of dogs




Suschinel R., Jaimes‑Mogollon AL., Fong Sim S., Ting W., Caceres-Tarazona JM., Alvarez-Valdez E., Rosero-Moreano M., Fethi-Diouani M., Chouihi E., Brebu M., Simion V., Barasona JA. and Ionescu R.




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Identification of putative volatile biomarkers of canine leishmaniasis in dog`s breath and hair employing a novel algorithm for automated chromatographic peak detection and matching

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Identification of putative volatile biomarkers of canine leishmaniasis in dog`s breath and hair employing a novel algorithm for automated chromatographic peak detection and matching



Participants:

Estonian University of Life SciencesInstitute of Veterinary Medicine and Animal Sciences. Estonian University of Life Sciences (EMU).

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

University of PamplonaGISM Group. Faculty of Engineering and Architecture. University of Pamplona.

Faculty of Resource Science and Technology. University Malaysia Sarawak.

Grupo de Investigación en Cromatografía y Técnicas Afnes (GICTA). Departamento de Química, Facultad de Ciencias Exactas y Naturales. Universidad de Caldas.

Institut Pasteur de Tunis (IPT).

“Petru Poni” Institute of Macromolecular Chemistry.

Faculty of Veterinary Medicine. Spiru Haret University.







FACTOR YEAR Q
3.800 2023

PMID: 39722103

ISSN: 1618-2642



TITLE: Identification of putative volatile biomarkers of canine leishmaniasis in dog`s breath and hair employing a novel algorithm for automated chromatographic peak detection and matching


JOURNAL: Anal Bioanal Chem


PUBLISHER: Springer Heidelberg


AUTHORS: Suschinel R., Jaimes‑Mogollon AL., Fong Sim S., Ting W., Caceres-Tarazona JM., Alvarez-Valdez E., Rosero-Moreano M., Fethi-Diouani M., Chouihi E., Brebu M., Simion V., Barasona JA. and Ionescu R.


José Ángel Barasona García-Arévalo

DOI: https://doi.org/10.1007/s00216-024-05691-1


CITE THIS PUBLICATION:

Suschinel R., Jaimes‑Mogollon AL., Fong Sim S., Ting W., Caceres-Tarazona JM., Alvarez-Valdez E., Rosero-Moreano M., Fethi-Diouani M., Chouihi E., Brebu M., Simion V., Barasona JA. and Ionescu R. Identification of putative volatile biomarkers of canine leishmaniasis in dog`s breath and hair employing a novel algorithm for automated chromatographic peak detection and matching. Analytical and Bioanalytical Chemistry. In Press. (A). ISSN: 1618-2642. DOI: 10.1007/s00216-024-05691-1


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