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In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study

Antimicrobial Agents and Chemotherapy publish this investigation article

February 5th, 2018

The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals poses an important safety concern for public health. With our in vivo broiler chicken infection study we investigated transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella (S.) Corvallis, without inducing antibiotic selection pressure. We evaluated dependency of the time point of inoculation on donor [S Corvallis (12-SA01738)] and a plasmid-free Salmonella spp. recipient [S Paratyphi B (dTa+), 13-SA01617] excretion by quantifying their excretion dynamics. Using S1-PFGE plasmid profiling we gained insight into the variability of native plasmid content among S Corvallis reisolates as well plasmid acquisition in S Paratyphi B (dTa+) and enterobacterial gut microflora. Whole genome sequencing enabled us an in-depth insight into microevolution of pRH-1238 plasmid in S Corvallis and enterobacterial recipient isolates. Our study revealed that the fecal excretion of avian native carbapenemase-producing S Corvallis is significantly higher and not hampered by S Paratyphi (dTa+). Acquisition of pRH-1238 in other Enterobacteriaceae and several transfer events of pRH-1238 plasmid to different E. coli sequence types and Klebsiella pneumoniae demonstrate interspecies broad-host range. Regardless of the microevolutionary structural deletions in pRH-1238, the single carbapenem resistance marker, blaNDM-1, was maintained on pRH-1238 throughout the trial. Furthermore, we showed the importance of the gut E. coli population as vector of pRH-1238. In a potential scenario of NDM-1-producing S Corvallis introduced into a broiler flock, the pRH-1238 plasmid can persist and spread to a broad-host range even in absence of antibiotic pressure




Hadziabdic S., Fischer J., Malorny B., Borowiak M., Guerra B., Kaesbohrer A., Gonzalez-Zorn B. and Szabo I.




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In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study

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In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study


Participants:

Federal Institute for Risk AssessmentDepartment of Biological Safety. Federal Institute for Risk Assessment (BfR).

European Food Safety AuthorityEuropean Food Safety Authority (EFSA).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Antimicrobial Agents and Chemotherapy
FACTOR YEAR Q
4.715 2018

NLMID: 315061

PMID: 29437622

ISSN: 0066-4804



TITLE: In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study


JOURNAL: Antimicrob. Agents Chemother.


NUMERACIÓN: 62(4)e02128-17:1-16


AÑO: 2018


PUBLISHER: American Society for Microbiology


AUTHORS: Hadziabdic S., Fischer J., Malorny B., Borowiak M., Guerra B., Kaesbohrer A., Gonzalez-Zorn B. and Szabo I.


VISAVET PARTICIPANTS


Bruno González Zorn

DOI: https://doi.org/ 10.1128/AAC.02128-17


CITE THIS PUBLICATION:

Hadziabdic S., Fischer J., Malorny B., Borowiak M., Guerra B., Kaesbohrer A., Gonzalez-Zorn B. and Szabo I. In vivo transfer and microevolution of avian native IncA/C2blaNDM-1-carrying plasmid pRH-1238 during a broiler chicken infection study. Antimicrobial Agents and Chemotherapy. 62(4)e02128-17:1-16. 2018. (A). ISSN: 0066-4804. DOI: 10.1128/AAC.02128-17


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