PLoS ONE publish this investigation article

December 3^rd, 2020

The increase in antimicrobial resistance in developed and developing countries is a global public health challenge. In this context β-lactamase production is a major contributing factor to resistance globally. The aim of this study was to determine the prevalence of phenotypic and genotypic extended spectrum β-lactamases (ESBLs) in 296 E. coli isolates recovered from diarrhoeic children younger than five years in Kano whose susceptibility profile against 7 antimicrobials had been determined. The E. coli isolates were subjected to double disc synergy test for phenotypic ESBLs detection and ESBL associated genes (blaCTX-M, blaTEM and blaSHV) were detected using conventional PCR. Phenotypically, 12.8% (38/296) E. coli isolates presented a ESBLs phenotype, with a significantly higher proportion in isolates from females compared with males (P-value = 0.024). blaCTX-M 73.3% and blaTEM 73.3% were the predominant resistance genes in the ESBLs positive E. coli (each detected in 22/30 isolates, of which 14 harboured both). In addition, 1/30 harboured blaCTX-M + blaTEM + blaSHV genes simultaneously. This study demonstrates the presence of ESBLs E. coli isolates in clinically affected children in Kano, and demonstrates the circulation of blaCTX-M and blaTEM associated with those phenotypes. Enactment of laws on prudent antibiotic use is urgently needed in Kano

Saka KH., Garcia-Soto S., Dabo NT., Lopez-Chavarrias V., Muhammad B., Ugarte-Ruiz M. and Alvarez J..

Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria

Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria

	Nigerian Stored Products Research Institute (NSPRI).
	Department of Microbiology. Bayero University Kano (BUK).
	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Department of Biological Sciences. Bayero University Kano (BUK).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).