Multi-Laboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens
Journal of Clinical Microbiology publish this investigation article
December 1st, 2017
A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M. caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotyping or qPCR) in each laboratory; liquid (MGIT or BacT/Alert) and/or solid (Stonebrink, Coletsos or Lowenstein-Jensen) cultures were tested. In comparison to spoligotyping of acid-fast positive MGIT cultures, percentage agreement between positive LFD and spoligotyping results was excellent in two UK laboratories (97.7-100%), but lower in the Spanish context (76%) where spoligotyping was applied to MGIT cultures previously confirmed to be positive for M. tuberculosis complex (MTBC) by qPCR. Certain spoligotypes of M. bovis and M. caprae were not detected by the LFD in Spanish MGIT cultures. Compared to qPCR confirmation, the percentage agreement between positive LFD and qPCR results was 42.3% and 50% for BacT/Alert and MGIT liquid cultures, respectively, and for solid cultures ranged from 11.1-89.2%, depending on solid medium employed (Coletsos 11.1%, Lowenstein-Jensen 55.6%, Stonebrinks 89.2%). Correlation between the novel LFD and BD MGIT TBc ID test results was excellent when 190 MGIT cultures were tested (r = 0.9791; P<0.0001), with the added benefit that M. bovis was differentiated from another MTBC species in one MGIT culture by the novel LFD. This multi-laboratory evaluation has demonstrated the novel LFD`s potential utility as a rapid test to confirm isolation of M. bovis and M. caprae from veterinary specimens following culture
Stewart LD., McCallan L., McNair J., McGoldrick A., Morris R., Moyen JL., de Juan L., Romero B., Alonso E., Parsons SDC., van Helden P., Araujo FR. and Grant IR.
| Institute for Global Food Security. School of Biological Sciences. Queen`s University Belfast. | |
| Veterinary Sciences Division (VSD). Agri-Food and Biosciences Institute (AFBI). | |
 | Animal and Plant Health Agency (APHA). |
| Laboratoire Départemental d´Analyse et de Recherche de la Dordogne. | |
 | Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Laboratorio Regional de Sanidad Animal. Consejería de Agricultura y Ganadería. Junta de Castilla y León. | |
| Division of Molecular Biology and Human Genetics. DST/NRF Centre of Excellence for Biomedical Tuberculosis Research. SAMRC Centre for TB Research. Faculty of Health Sciences. Stellenbosch University (SUN). | |
 | Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento. |
