Oral communication in 23 Congreso Internacional de la Sociedad de Veterinarios Porcinos

June 8^th, 2014

Gimenez-Lirola LG., Mur L., Rivera B., Wang C., Rowland R., Harris DL., Gallardo C., Arias M., Zimmerman JJ. and Sanchez-Vizcaino JM.

Introduction
Serology has been been widely used in ASFV control programs in the Iberian Peninsula and Sardinia as a tool for the detection of ASFV carrier animals. Several ASFV structural and non-structural proteins have being identified as candidate antigens for serological tests (1,2). Among these, structural proteins p30, p54, and p72 are the best described, most highly studied, and most
widely used in commercial ASFV serum antibody
ELISAs.
Although ELISA is the most widely used tool for screening purposes, it is limited to measuring a single biomarker at a time. The development of spectrally distinguishable fluorescent beads-based technology
(Luminex® Corp, Austin, TX) make it possible to detect antibody against different antigen targets in a single reaction using antigen-coupled beads (3). The purpose of
this study was to evaluate the serum antibody response
against three major ASFV proteins (p30, p72 and p54) in a single assay using a multiplex fluorescent microbead based immunoassay (FMIA)

	Servicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	University of Iowa.
	Kansas State University (KSU).
	Centro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Ministerio de Ciencia e Innovación.
	Harrisvaccines, Inc.