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Multiplex Luminex assay for detection of antibodies against three major proteins of ASFV

Oral communication in 23 Congreso Internacional de la Sociedad de Veterinarios Porcinos

June 8th, 2014

Gimenez-Lirola LG., Mur L., Rivera B., Wang C., Rowland R., Harris DL., Gallardo C., Arias M., Zimmerman JJ. and Sanchez-Vizcaino JM.

Introduction
Serology has been been widely used in ASFV control programs in the Iberian Peninsula and Sardinia as a tool for the detection of ASFV carrier animals. Several ASFV structural and non-structural proteins have being identified as candidate antigens for serological tests (1,2). Among these, structural proteins p30, p54, and p72 are the best described, most highly studied, and most
widely used in commercial ASFV serum antibody
ELISAs.
Although ELISA is the most widely used tool for screening purposes, it is limited to measuring a single biomarker at a time. The development of spectrally distinguishable fluorescent beads-based technology
(Luminex® Corp, Austin, TX) make it possible to detect antibody against different antigen targets in a single reaction using antigen-coupled beads (3). The purpose of
this study was to evaluate the serum antibody response
against three major ASFV proteins (p30, p72 and p54) in a single assay using a multiplex fluorescent microbead based immunoassay (FMIA)




Participants:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

University of Iowa.

Kansas State UniversityKansas State University (KSU).

Centro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Ministerio de Ciencia e Innovación.

Harrisvaccines, Inc.


Link to 23nd International Pig Veterinary Society Congress





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23nd International Pig Veterinary Society Congress


Jun 8th - September 11st, 2014
Cancún
Mexico

TITLE: Multiplex Luminex assay for detection of antibodies against three major proteins of ASFV


TYPE: Oral communication


AUTHORS: Gimenez-Lirola LG., Mur L., Rivera B., Wang C., Rowland R., Harris DL., Gallardo C., Arias M., Zimmerman JJ. and Sanchez-Vizcaino JM.


VISAVET PARTICIPANTS


3rd
Belén Rivera Arroyo
Last
José Manuel Sánchez-Vizcaíno Rodríguez

DATE: June 8th, 2014


CITE THIS COMMUNICATION:

Gimenez-Lirola LG., Mur L., Rivera B., Wang C., Rowland R., Harris DL., Gallardo C., Arias M., Zimmerman JJ. and Sanchez-Vizcaino JM. Multiplex Luminex assay for detection of antibodies against three major proteins of ASFV. 23nd International Pig Veterinary Society Congress, International Pig Veterinary Society, Cancún, Mexico, June 8th, 2014. (Oral communication)


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