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August, 2017 news

August 30th, 2017

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Veterinary Parasitology

Application of a specific quantitative real-time PCR (qPCR) to identify Leishmania infantum DNA in spleen, skin and hair samples of wild Leporidae

Veterinary Parasitology publish this investigation article

The aim of this study was to compare a quantitative real-time PCR (qPCR) validated for the detection of Leishmania infantum in dogs with a nested PCR but in wild Leporidae. Additionally, L. infantum results from indirect immunofluorescent antibody test (IFAT) and in vitro culture were also compared with qPCR. Different samples (spleen, skin and hair) recovered from 224 European rabbits and 70 Iberian hares from two green areas of Madrid Council were analysed for the detection of L. infantum. The presence of Leishmania kDNA was detected by qPCR in 58 out of 221 (26.24%), 162 out of 203 (79.8%) … Read more

August 12nd, 2017

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El Mundo TV

El uso de colistina en la salud animal y humana

Interview in El Mundo TV of El Mundo

August 12nd, 2017

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El Mundo TV

El microbiólogo Bruno González Zorn explica cómo una bacteria se convierte en resistente

Interview in El Mundo TV of El Mundo

August 7th, 2017

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Frontiers in veterinary science

Oral Vaccination with Heat-Inactivated Mycobacterium bovis Does Not Interfere with the Antemortem Diagnostic Techniques for Tuberculosis in Goats

Frontiers in veterinary science publish this investigation article

Vaccination against tuberculosis (TB) is prohibited in cattle or other species subjected to specific TB eradication campaigns, due to the interference that it may cause with the official diagnostic tests. However, immunization with a heat-inactivated (HI) Mycobacterium bovis vaccine via the oral route has been suggested to overcome this issue. In this study, the main goal was to assess the interference of the HI vaccine by different routes of administration using a previous vaccination and re-vaccination (boosting) protocol. TB-free kid goats were divided into three groups: oral (n = 16), intr… Read more

August 3rd, 2017

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Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015

Investigation published in Eurosurveillance

Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.… Read more

August 3rd, 2017

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VISAVET Outreach Journal

EIA: Equine Infectious Anemia

New online article published in VISAVET Outreach Journal

Equine Infectious Anemia (EIA) or swamp fever is a chronic infectious disease of equids caused by a lentivirus of the Retroviridae family. In this article we summarise the most important features of the transmission, clinical signs, diagnosis and control measures to be implemented once the diagnosis has been confirmed, as well as the present situation of the disease in Spain.… Read more

August 1st, 2017

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Revue Scientifique et Technique

Technological advances in veterinary diagnostics: opportunities to deploy rapid decentralised tests to detect pathogens affecting livestock

Revue Scientifique et Technique publish this investigation article

Sustainable food production capable of feeding a growing human population is a significant global challenge, and is a priority encompassed within the United Nations Millennium Development Goal to `eradicate extreme poverty and hunger`. Infectious diseases reduce the productivity of farm animals, and the globalised trade of animals and their products increases the threat of disease incursion. Accurate and rapid diagnostic tests are an essential component of contingency plans to detect, control and eradicate such diseases. Diagnosis involves a `pipeline` that normally starts with clinical suspic… Read more

August 1st, 2017

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The Veterinary Record

Isolation of Brachyspira species from farmed wild boar in Spain

Investigation article published in The Veterinary Record

Bacteria belonging to the genus Brachyspira are gram-negative, anaerobic, spirochaetes adapted to occupy specialised niches in the large intestines of animals, including swine.1 Brachyspira species cause important digestive diseases such as swine dysentery (Brachyspira hyodysenteriae) and porcine intestinal spirochetosis (Brachyspira pilosicoli), which have a significant economic impact on the swine industry. Spain has major problems associated with swine dysentery, as demonstrated by Carvajal and others, who showed that more than 30% of Spanish farms and 12% of porcine faecal specimens tested… Read more

August 1st, 2017

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International Journal of Antimicrobial Agents

Escherichia coli ST167 carrying plasmid mobilisable mcr-1 and blaCTX-M-15 resistance determinants isolated from a human respiratory infection

Investigation published in International Journal of Antimicrobial Agents

The emergence of polymyxin resistance in Enterobacteriaceae is a matter of major concern and has instigated the recommendation for its surveillance since it compromises the use of colistin, a last-resort antibiotic.… Read more

August 1st, 2017

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LWT - Food Science and Technology

Effect of seafood peptones on biomass and metabolic activity by Enterococcus faecalis DM19

Investigation published in LWT - Food Science and Technology

Eight seafood protein hydrolysates (SPHs) obtained from squid, shrimp and fish gelatin were incorporated as substitutes of peptones in culture media in order to evaluate its effect on survival and metabolic activity (lactic acid, acetic acid and bacteriocins production) of Enterococcus faecalis DM19. The substitution of commercial peptones in culture media by either a shrimp hydrolysate prepared with Protamex, or by squid protein hydrolysates prepared with Esperase or Alkaline protease, stimulated E. faecalis DM19 growth up to 16%. The incorporation of SPHs, mainly from shrimp, in the culture … Read more

August 1st, 2017

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Transboundary and Emerging Diseases

Serological Surveillance and Direct Field Searching Reaffirm the Absence of Ornithodoros Erraticus Ticks Role in African Swine Fever Cycle in Sardinia

Investigation published in Transboundary and Emerging Diseases

African swine fever (ASF), one of the most important diseases of swine, has been endemic in the Italian island of Sardinia for more than 35 years. During these decades, several strategies and eradication efforts have been implemented in the island with limited success. Strong climatic and ecological similarities exist between Sardinia and one area of the Iberian Peninsula where Ornithodoros erraticus ticks were involved in the persistence of ASF from 1960 to 1995. This fact leads to the hypothesis that, potentially, Ornithodoros ticks could be also involved in the ASF cycle in Sardinia, thus a… Read more

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