Oral communication in Seminarios VISAVET 2024

September 2^nd, 2024

Rebollada A.

In-situ hybridization (ISH) has emerged as a pivotal technique in veterinary diagnostic pathology, offering precise localization and characterization of nucleic acids within tissue samples. This presentation explores the applications of ISH in veterinary medicine, emphasizing its utility in detecting viral, bacterial, and protozoal infections, as well as its role in identifying genetic aberrations and gene expression patterns associated with neoplastic and developmental disorders. The integration of ISH with traditional histopathology enhances diagnostic accuracy, aids in understanding disease pathogenesis, and facilitates targeted therapeutic strategies in veterinary practice. Specifically, ISH enables veterinarians to pinpoint the presence and distribution of pathogens directly within tissues, crucial for timely diagnosis and management of infectious diseases. Moreover, ISH techniques such as fluorescence in-situ hybridization (FISH) and chromogenic in-situ hybridization (CISH) offer distinct advantages in visualizing nucleic acids, making them indispensable tools in the armamentarium of veterinary pathologists. This presentation highlights the advancements and challenges of ISH, positioning it as a crucial tool for comprehensive veterinary pathology investigations, poised to further enhance diagnostic precision and therapeutic outcomes in veterinary medicine

	Section of Anatomic Pathology. College of Veterinary Medicine. Cornell University.
	Servicio de Patología y Veterinaria Forense (SAP). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).