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Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants

Investigation article published in BMC Microbiology

October 7th, 2009

Mycobacterium avium subspecies paratuberculosis (Map) causes an infectious chronic enteritis (paratuberculosis or Johne`s disease) principally of ruminants. The epidemiology of Map is poorly understood, particularly with respect to the role of wildlife reservoirs and the controversial issue of zoonotic potential (Crohn`s disease). Genotypic discrimination of Map isolates is pivotal to descriptive epidemiology and resolving these issues. This study was undertaken to determine the genetic diversity of Map, enhance our understanding of the host range and distribution and assess the potential for interspecies transmission. RESULTS: 164 Map isolates from seven European countries representing 19 different host species were genotyped by standardized IS900 - restriction fragment length polymorphism (IS900-RFLP), pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphisms (AFLP) and mycobacterial interspersed repeat unit-variable number tandem repeat (MIRU-VNTR) analyses. Six PstI and 17 BstEII IS900-RFLP, 31 multiplex [SnaBI-SpeI] PFGE profiles and 23 MIRU-VNTR profiles were detected. AFLP gave insufficient discrimination of isolates for meaningful genetic analysis. Point estimates for the discrimination indices calculated for the individual typing techniques were in the range of 0.636 - 0.664 but a combination of all three methods increased the discriminative power to 0.879, sufficient for investigating transmission dynamics. Two predominant strain types were detected across Europe with all three typing techniques. Evidence for interspecies transmission between wildlife and domestic ruminants on the same property was demonstrated in four cases, between wildlife species on the same property in two cases and between different species of domestic livestock on one property. CONCLUSIONS: The results of this study showed that it is necessary to use multiple genotyping techniques targeting different sources of genetic variation to obtain the level of discrimination necessary to investigate transmission dynamics and trace the source of Map infections. Furthermore, the combination of genotyping techniques may depend on the geographical location of the population to be tested. Identical genotypes were obtained from Map isolated from different host species co-habiting on the same property strongly suggesting that interspecies transmission occurs. Interspecies transmission of Map between wildlife species and domestic livestock on the same property provides further evidence to support a role for wildlife reservoirs of infection




Stevenson K., Alvarez J., Biet F., de Juan L., Denham S., Dimareli Z., Dohmann K., Gerlach GF., Heron I., Kopecna M., May L., Pavlik I., Sharp JM., Thibault V., Willemsen P., Zadoks R. and Greig A.




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Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants

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Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants


Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Wageningen UniversityCentral Veterinary Institute. Wageningen University (UR).

Institut National de la Recherche AgronomiqueCentre de Tours. Institut National de la Recherche Agronomique (INRA).

Veterinary Research Institute of Thessaloniki. National Agricultural Research Foundation (NAGREF).

Institut für Mikrobiologie. Tierärztliche Hochschule Hannover.

Veterinary Research Institute. Czechoslovak Academy of Agricultural Sciences.

Veterinary Laboratories AgencyVeterinary Laboratories Agency.

Laboratoire Microorganismes : Génome et Environnement. Université Blaise Pascal, Clermont-Ferrand (UBP).

Scottish Crop Research Institute (SCRI).

Pentlands Science Park. Moredun Research Institute.







BMC Microbiology
FACTOR YEAR Q
2.890 2009

NLMID: 100966981

PMID: 19811631

ISSN: 1471-2180



TITLE: Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants


JOURNAL: BMC Microbiol.


NUMERACIÓN: 9:212


AÑO: 2009


PUBLISHER: BioMed Central


AUTHORS: Stevenson K., Alvarez J., Biet F., de Juan L., Denham S., Dimareli Z., Dohmann K., Gerlach GF., Heron I., Kopecna M., May L., Pavlik I., Sharp JM., Thibault V., Willemsen P., Zadoks R. and Greig A.


VISAVET PARTICIPANTS


2nd
Julio Álvarez Sánchez
5th
Lucía de Juan Ferré

DOI: https://doi.org/ 10.1186/1471-2180-9-212


CITE THIS PUBLICATION:

Stevenson K., Alvarez J., Biet F., de Juan L., Denham S., Dimareli Z., Dohmann K., Gerlach GF., Heron I., Kopecna M., May L., Pavlik I., Sharp JM., Thibault V., Willemsen P., Zadoks R. and Greig A. Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants. BMC Microbiology. 9:212. 2009. (A). ISSN: 1471-2180. DOI: 10.1186/1471-2180-9-212


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