Investigation article published in Microbiological Research

March 15^th, 2008

Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes

Hassan AA., Vossen A., Lammler C., Siebert U. and Fernandez-Garayzabal JF..

PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae

PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae

	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Institut für Pharmakologie und Toxikologie. Justus-Liebig-Universität Gießen (JLU).
	Institut für Tierärztliche Nahrungsmittelkunde (IfTN). Justus-Liebig-Universität Gießen (JLU).
	Rhein–Sieg–Kreis.
	Forschung-und Technologiezentrum Westküste. Christian-Albrechts-Universität zu Kiel (CAU).