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PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae

Investigation article published in Microbiological Research

March 15th, 2008

Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes




Hassan AA., Vossen A., Lammler C., Siebert U. and Fernandez-Garayzabal JF..




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PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae

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PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae


Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Justus-Liebig-Universität GießenInstitut für Pharmakologie und Toxikologie. Justus-Liebig-Universität Gießen (JLU).

Justus-Liebig-Universität GießenInstitut für Tierärztliche Nahrungsmittelkunde (IfTN). Justus-Liebig-Universität Gießen (JLU).

Rhein–Sieg–Kreis.

Forschung-und Technologiezentrum Westküste. Christian-Albrechts-Universität zu Kiel (CAU).







FACTOR YEAR Q
2.054 2008

NLMID: 9437794

PMID: 16793248

ISSN: 0944-5013



TITLE: PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae


JOURNAL: Microbiol. Res.


NUMERACIÓN: 163(2):132-5


AÑO: 2008


PUBLISHER: G. Fischer


AUTHORS: Hassan AA., Vossen A., Lammler C., Siebert U. and Fernandez-Garayzabal JF..


VISAVET PARTICIPANTS


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/ 10.1016/j.micres.2006.01.017


CITE THIS PUBLICATION:

Hassan AA., Vossen A., Lammler C., Siebert U. and Fernandez-Garayzabal JF. PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae. Microbiological Research. 163(2):132-5. 2008. (A). ISSN: 0944-5013. DOI: 10.1016/j.micres.2006.01.017


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