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Performance and Agreement Between WGS Variant Calling Pipelines Used for Bovine Tuberculosis Control: Toward International Standardization

Frontiers in veterinary science publish this investigation article

December 14th, 2021

Whole genome sequencing (WGS) and allied variant calling pipelines are a valuable tool for the control and eradication of infectious diseases, since they allow the assessment of the genetic relatedness of strains of animal pathogens. In the context of the control of tuberculosis (TB) in livestock, mainly caused by Mycobacterium bovis, these tools offer a high-resolution alternative to traditional molecular methods in the study of herd breakdown events. However, despite the increased use and efforts in the standardization of WGS methods in human tuberculosis around the world, the application of these WGS-enabled approaches to control TB in livestock is still in early development. Our study pursued an initial evaluation of the performance and agreement of four publicly available pipelines for the analysis of M. bovis WGS data (vSNP, SNiPgenie, BovTB, and MTBseq) on a set of simulated Illumina reads generated from a real-world setting with high TB prevalence in cattle and wildlife in the Republic of Ireland. The overall performance of the evaluated pipelines was high, with recall and precision rates above 99% once repeat-rich and problematic regions were removed from the analyses. In addition, when the same filters were applied, distances between inferred phylogenetic trees were similar and pairwise comparison revealed that most of the differences were due to the positioning of polytomies. Hence, under the studied conditions, all pipelines offer similar performance for variant calling to underpin real-world studies of M. bovis transmission dynamics




Lorente-Leal V., Farrell D., Romero B., Alvarez J., de Juan L. and Gordon SV.




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Performance and Agreement Between WGS Variant Calling Pipelines Used for Bovine Tuberculosis Control: Toward International Standardization

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Performance and Agreement Between WGS Variant Calling Pipelines Used for Bovine Tuberculosis Control: Toward International Standardization



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

University College DublinUCD School of Agriculture, Food Science & Veterinary Medicine. University College Dublin (UCD).







Frontiers in veterinary science
FACTOR YEAR Q
3.412 2020

NLMID: 101666658

PMID: 34970617

ISSN: 2297-1769



TITLE: Performance and Agreement Between WGS Variant Calling Pipelines Used for Bovine Tuberculosis Control: Toward International Standardization


JOURNAL: Front Vet Sci


NUMERACIÓN: 8:780018


AÑO: 2021


PUBLISHER: Lausanne : Frontiers Media S.A


AUTHORS: Lorente-Leal V., Farrell D., Romero B., Alvarez J., de Juan L. and Gordon SV.


VISAVET PARTICIPANTS


First
Victor Lorente Leal
3rd
Beatriz Romero Martínez
4th
Julio Álvarez Sánchez
5th
Lucía de Juan Ferré

DOI: https://doi.org/10.3389/fvets.2021.780018


CITE THIS PUBLICATION:

Lorente-Leal V., Farrell D., Romero B., Alvarez J., de Juan L. and Gordon SV. Performance and Agreement Between WGS Variant Calling Pipelines Used for Bovine Tuberculosis Control: Toward International Standardization. Frontiers in veterinary science. 8:780018. 2021. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2021.780018


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