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Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms

Veterinary Immunology and Immunopathology publish this investigation article

April 8th, 2022

Bovine tuberculosis (bTB) control programs can be improved by combined use of tests for humoral and cell-mediated immune responses targeting multiple biomarkers of Mycobacterium bovis. To further the diagnostic benefits of this approach, we used Dual Path Platform (DPP) technology to test sera from cattle with naturally acquired bTB in the United States (US) and Spain for the presence of M. bovis antigen, IgM and/or IgG antibodies to MPB70/MPB83 fusion antigen in conjunction with tuberculin skin tests (TST) or interferon-gamma release assays (IGRA). When TST was complemented with detection of IgM and IgG antibodies, the diagnostic sensitivity increased from 85.4% to 95.1% in the US and from 64.2% to 81.5% in Spain. Likewise, adding the DPP assays enhanced IGRA diagnostic sensitivity from 82.7% to 93.8% in Spain. Detection of circulating M. bovis antigen showed added value when used in combination with the DPP antibody assays but it was limited when analyzed in the context of TST or IGRA results. Present findings support the benefits of a multi-test approach for the ante-mortem diagnosis of bTB in cattle




Sridhara AA., Johnathan-Lee A., Elahi R., Sikar-Gang A., Lambotte P., Esfandiari J., de Juan L., Gortazar C., Marpe BN., Thacker TC., Palmer MV., Waters WR. and Lyashchenko K.




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Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms

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Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms



Participants:

Chembio Diagnostic Systems IncChembio Diagnostic Systems Inc.

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

United States Department of AgricultureVeterinary Services. Animal and Plant Health Inspection. United States Department of Agriculture.

United States Department of AgricultureAgricultural Research Service. United States Department of Agriculture.







Veterinary Immunology and Immunopathology
FACTOR YEAR Q
2.046 2020

NLMID: 8002006

PMID: 35439655

ISSN: 0165-2427



TITLE: Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms


JOURNAL: Vet. Immunol. Immunopathol.


NUMERACIÓN: 248:110419


AÑO: 2022


PUBLISHER: Elsevier Scientific


AUTHORS: Sridhara AA., Johnathan-Lee A., Elahi R., Sikar-Gang A., Lambotte P., Esfandiari J., de Juan L., Gortazar C., Marpe BN., Thacker TC., Palmer MV., Waters WR. and Lyashchenko K.


VISAVET PARTICIPANTS


Lucía de Juan Ferré

DOI: https://doi.org/10.1016/j.vetimm.2022.110419


CITE THIS PUBLICATION:

Sridhara AA., Johnathan-Lee A., Elahi R., Sikar-Gang A., Lambotte P., Esfandiari J., de Juan L., Gortazar C., Marpe BN., Thacker TC., Palmer MV., Waters WR. and Lyashchenko K. Potential for improved detection of bovine tuberculosis by targeting combined blood biomarkers in multi-test algorithms. Veterinary Immunology and Immunopathology. 248:110419. 2022. (A). ISSN: 0165-2427. DOI: 10.1016/j.vetimm.2022.110419


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