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Prevalence and molecular characterization of New Delhi metallo-Beta-lactamases NDM-1, NDM-5, NDM-6 and NDM-7 in multidrug-resistant Enterobacteriaceae from India

Investigation article published in International Journal of Antimicrobial Agents

July 1st, 2014

The growing prevalence of carbapenem resistance in Enterobacteriaceae worldwide is a major concern. New Delhi metallo-Beta-lactamase (NDM)-mediated carbapenem resistance has been identified in Enterobacteriaceae from numerous countries including those of the Indian subcontinent. Currently, seven NDM β-lactamase variants (NDM-1 to -7) have been identified. This study evaluated the detection and molecular characterisation of NDM variants in Enterobacteriaceae at a tertiary care hospital in India. A total of 464 isolates were tested; 57 (12.3%) were resistant or showed reduced susceptibility to imipenem and meropenem. All carbapenem-resistant isolates were blaNDM-positive by PCR, but 13 isolates bore variants that differed in sequence from blaNDM-1. NDM-5, NDM-6 and NDM-7 were identified in two, eight and three isolates, respectively. blaNDM variants were located on plasmids of >100kb with IncF, IncA/C and untypeable replicon types. Genes encoding the 16S rRNA methyltransferases RmtB, RmtC and ArmA as well as those for AmpC β-lactamases were also located on the same plasmids as blaNDM in different combinations. The prevalence of NDM-5 to -7 variants was significantly higher in Escherichia coli (P=0.015) and they were more frequently isolated from the urology ward (P=0.037) than NDM-1. The mortality rate was comparable between patients infected with isolates positive for blaNDM-1 and blaNDM variants [25% (11/44) vs. 23% (3/13)]. Expression of blaNDM variants in E. coli using the same promoter showed that NDM-7 conferred higher resistance to imipenem. The diverse genotypic features of blaNDM indicate rapid evolution of NDM resulting from their wide spread in the Indian subcontinent




Rahman M., Shukla S., Prasad KN., Ovejero CM., Pati BK., Triphati A., Singh A., Srivastava AK. and Gonzalez-Zorn B..




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Prevalence and molecular characterization of New Delhi metallo-Beta-lactamases NDM-1, NDM-5, NDM-6 and NDM-7 in multidrug-resistant Enterobacteriaceae from India

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Prevalence and molecular characterization of New Delhi metallo-Beta-lactamases NDM-1, NDM-5, NDM-6 and NDM-7 in multidrug-resistant Enterobacteriaceae from India


Participants:

Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS).

Faculty of Applied Sciences. Integral University.

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







International Journal of Antimicrobial Agents
FACTOR YEAR Q
4.296 2014

NLMID: 9111860

PMID: 24831713

ISSN: 0924-8579



TITLE: Prevalence and molecular characterization of New Delhi metallo-Beta-lactamases NDM-1, NDM-5, NDM-6 and NDM-7 in multidrug-resistant Enterobacteriaceae from India


JOURNAL: Int. J. Antimicrob. Agents


NUMERACIÓN: 44(1):30-7


AÑO: 2014


PUBLISHER: Elsevier Science Publishers


AUTHORS: Rahman M., Shukla S., Prasad KN., Ovejero CM., Pati BK., Triphati A., Singh A., Srivastava AK. and Gonzalez-Zorn B..


VISAVET PARTICIPANTS


Bruno González Zorn

DOI: https://doi.org/ 10.1016/j.ijantimicag.2014.03.003


CITE THIS PUBLICATION:

Rahman M., Shukla S., Prasad KN., Ovejero CM., Pati BK., Triphati A., Singh A., Srivastava AK. and Gonzalez-Zorn B. Prevalence and molecular characterization of New Delhi metallo-Beta-lactamases NDM-1, NDM-5, NDM-6 and NDM-7 in multidrug-resistant Enterobacteriaceae from India. International Journal of Antimicrobial Agents. 44(1):30-7. 2014. (A). ISSN: 0924-8579. DOI: 10.1016/j.ijantimicag.2014.03.003


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