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Profile and resistance levels of 136 integron resistance genes

Investigation published in npj Antimircobials and Resistance

October 11st, 2023

Integrons have played a major role in the rise and spread of multidrug resistance in Gram-negative pathogens and are nowadays commonplace among clinical isolates. These platforms capture, stockpile, and modulate the expression of more than 170 antimicrobial resistance cassettes (ARCs) against most clinically-relevant antibiotics. Despite their importance, our knowledge on their profile and resistance levels is patchy, because data is scattered in the literature, often reported in different genetic backgrounds and sometimes extrapolated from sequence similarity alone. Here we have generated a collection of 136 ARCs against 8 antibiotic families and disinfectants. Cassettes are cloned in a vector designed to mimic the genetic environment of a class 1 integron, and transformed in Escherichia coli. We have measured the minimal inhibitory concentration (MIC) to the most relevant molecules from each antibiotic family. With more than 500 MIC values, we provide an exhaustive and comparable quantitation of resistance conferred by ARCs. Our data confirm known resistance trends and profiles while revealing important differences among closely related genes. We have also detected genes that do not confer the expected resistance, to the point of challenging the role of the whole family of qac genes in resistance against disinfectants. Our work provides a detailed characterization of integron resistance genes at-a-glance




Hipolito A., Garcia-Pastor L., Vergara-Gonzalez E., Jove T. and Escudero JA..




See this article
Profile and resistance levels of 136 integron resistance genes





Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Bases Moleculares de Adaptación. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Institut national de la santé et de la recherche médicaleInstitut national de la santé et de la recherche médicale (INSERM).

Université de LimogesUniversité de Limoges (UL).







npj Antimircobials and Resistance
FACTOR YEAR Q
0.000 2023

ISSN: 2731-8745



TITLE: Profile and resistance levels of 136 integron resistance genes


JOURNAL: npj Antimicrobials and Resistance


NUMERACIÓN: 1:13


AÑO: 2023


PUBLISHER: Nature


AUTHORS: Hipolito A., Garcia-Pastor L., Vergara-Gonzalez E., Jove T. and Escudero JA..


José Antonio Escudero García-Calderón

DOI: https://doi.org/10.1038/s44259-023-00014-3


CITE THIS PUBLICATION:

Hipolito A., Garcia-Pastor L., Vergara-Gonzalez E., Jove T. and Escudero JA. Profile and resistance levels of 136 integron resistance genes. npj Antimircobials and Resistance. 1:13. 2023. (A). ISSN: 2731-8745. DOI: 10.1038/s44259-023-00014-3


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