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Protection against Tuberculosis in Eurasian Wild Boar Vaccinated with Heat-Inactivated Mycobacterium bovis

Investigation published in PLoS ONE

September 14th, 2011

Tuberculosis (TB) caused by Mycobacterium bovis and closely related members of the Mycobacterium tuberculosis complex continues to affect humans and animals worldwide and its control requires vaccination of wildlife reservoir species such as Eurasian wild boar (Sus scrofa). Vaccination efforts for TB control in wildlife have been based primarily on oral live BCG formulations. However, this is the first report of the use of oral inactivated vaccines for controlling TB in wildlife. In this study, four groups of 5 wild boar each were vaccinated with inactivated M. bovis by the oral and intramuscular routes, vaccinated with oral BCG or left unvaccinated as controls. All groups were later challenged with a field strain of M. bovis. The results of the IFN-gamma response, serum antibody levels, M. bovis culture, TB lesion scores, and the expression of C3 and MUT genes were compared between these four groups. The results suggested that vaccination with heat-inactivated M. bovis or BCG protect wild boar from TB. These results also encouraged testing combinations of BCG and inactivated M. bovis to vaccinate wild boar against TB. Vaccine formulations using heat-inactivated M. bovis for TB control in wildlife would have the advantage of being environmentally safe and more stable under field conditions when compared to live BCG vaccines. The antibody response and MUT expression levels can help differentiating between vaccinated and infected wild boar and as correlates of protective response in vaccinated animals. These results suggest that vaccine studies in free-living wild boar are now possible to reveal the full potential of protecting against TB using oral M. bovis inactivated and BCG vaccines




Garrido JM., Sevilla I., Beltran-Beck B., Minguijon E., Ballesteros C., Galindo RC., Boadella M., Lyashchenko KP., Romero B., Geijo MV., Ruiz-Fons F., Aranaz A., Juste R., Vicente J., de la Fuente J. and Gortazar C.




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Protection against Tuberculosis in Eurasian Wild Boar Vaccinated with Heat-Inactivated Mycobacterium bovis

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Protection against Tuberculosis in Eurasian Wild Boar Vaccinated with Heat-Inactivated Mycobacterium bovis



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno VascoNeiker-Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario. Gobierno Vasco.

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Chembio Diagnostic Systems IncChembio Diagnostic Systems Inc.

Oklahoma State UniversityDepartament of Veterinary Pathobiology. Center for Veterinary Health Sciences (CVHS). Oklahoma State University (OSU).







PLoS ONE
FACTOR YEAR Q
4.092 2011

NLMID: 101285081

PMID: 21935486

ISSN: 1932-6203



TITLE: Protection against Tuberculosis in Eurasian Wild Boar Vaccinated with Heat-Inactivated Mycobacterium bovis


JOURNAL: PLoS ONE


NUMERACIÓN: 6(9):e24905


AÑO: 2011


PUBLISHER: Public Library Science


AUTHORS: Garrido JM., Sevilla I., Beltran-Beck B., Minguijon E., Ballesteros C., Galindo RC., Boadella M., Lyashchenko KP., Romero B., Geijo MV., Ruiz-Fons F., Aranaz A., Juste R., Vicente J., de la Fuente J. and Gortazar C.


VISAVET PARTICIPANTS


Beatriz Romero Martínez

DOI: https://doi.org/10.1371/journal.pone.0024905


CITE THIS PUBLICATION:

Garrido JM., Sevilla I., Beltran-Beck B., Minguijon E., Ballesteros C., Galindo RC., Boadella M., Lyashchenko KP., Romero B., Geijo MV., Ruiz-Fons F., Aranaz A., Juste R., Vicente J., de la Fuente J. and Gortazar C. Protection against Tuberculosis in Eurasian Wild Boar Vaccinated with Heat-Inactivated Mycobacterium bovis. PLoS ONE. 6(9):e24905. 2011. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0024905


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