Investigation article published in Transboundary and Emerging Diseases

January 14^th, 2026

Reports of brucellosis in free-ranging cetaceans are increasing worldwide, particularly in the Mediterranean Sea. To enhance diagnostic accuracy and epidemiological understanding of cetacean brucellosis in the Western Mediterranean Sea, we analyzed bacteriological, serological, and molecular data from 30 cetaceans belonging to three different species stranded along the coast of the Valencian Community (Spain) between 2011 and 2021. Brucella ceti infection was confirmed by bacteriological isolation in 14 animals (46.7%) and by genus-specific qPCR in 15 cases (50%), with some discrepancies between methods. When feasible, serological analyses were performed using a commercial blocking ELISA (bELISA) and/or the Rose Bengal agglutination test (RBT). In the absence of ELISA tests properly validated for its use in marine mammals, we assessed the optimum dilution and cut-off of this ELISA kit using panels of gold-standard sera from culture-positive and brucellosis-free dolphins. From a pathological perspective, 12 infected animals showed moderate to severe meningoencephalitis or meningoencephalomyelitis with lymphoplasmacytic infiltration. Additionally, whole-genome sequencing (WGS) enabled the identification of two sequence types (STs), ST26 and ST49, indicating phylogenetic divergence. Our findings provide new insights into the phylogenetics of B. ceti and highlight the particular susceptibility of striped dolphins to this bacterium. The study also evidences the need for proper validation of the indirect diagnostic methods used for surveillance and seroepidemiological studies of brucellosis in marine mammals

Vargas-Castro I., Andres-Barranco S., Crespo-Picazo JL., Torre-Fuentes L., Jimenez-Martinez MA., Hernandez M., Arbelo M., Alvarez J., Munoz PM., Marco-Cabedo V., de Miguel MJ., López-Gutiérrez D., Munoz-Baquero M., Garcia-Parraga D. and Barasona JA..

Towards Integrated Surveillance of Marine Brucellosis: Diagnostic and Phylogenetic Assessment of Brucella ceti in Stranded Dolphins of the Western Mediterranean Sea

Towards Integrated Surveillance of Marine Brucellosis: Diagnostic and Phylogenetic Assessment of Brucella ceti in Stranded Dolphins of the Western Mediterranean Sea

	Departamento de Sanidad Animal. Departamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Servicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA). Departamento de Industria e Innovación. Gobierno de Aragón.
	Research Department. Oceanogràfic. Ciudad de las Artes y las Ciencias. Generalitat Valenciana.
	Departamento de 25 Anatomía Patológica, Microbiología, Medicina Preventiva y Salud Pública, Medicina Legal y Forense. Facultad de Medicina. Universidad de Valladolid (UVa).
	Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA). Facultad de Veterinaria. Universidad de Las Palmas de Gran Canaria (ULPGC).