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Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration

Cell metabolism publish this investigation article

January 7th, 2020

Life on Earth emerged in a hydrogen sulfide (H2S)-rich environment eons ago and with it protein persulfidation mediated by H2S evolved as a signaling mechanism. Protein persulfidation (S-sulfhydration) is a post-translational modification of reactive cysteine residues, which modulate protein structure and/or function. Persulfides are difficult to label and study due to their reactivity and similarity with cysteine. Here, we report a facile strategy for chemoselective persulfide bioconjugation using dimedone-based probes, to achieve highly selective, rapid, and robust persulfide labeling in biological samples with broad utility. Using this method, we show persulfidation is an evolutionarily conserved modification and waves of persulfidation are employed by cells to resolve sulfenylation and prevent irreversible cysteine overoxidation preserving protein function. We report an age-associated decline in persulfidation that is conserved across evolutionary boundaries. Accordingly, dietary or pharmacological interventions to increase persulfidation associate with increased longevity and improved capacity to cope with stress stimuli




Zivanovic J., Kouroussis E., Kohl JB., Adhikari B., Bursac B., Schott-Roux S., Petrovic D., Miljkovic JL., Thomas-Lopez D., Jung Y., Miler M., Mitchell S., Milosevic V., Gomes JE., Benhar M., Gonzalez-Zorn B., Ivanovic-Burmazovic I., Torregrossa R., Mitchell JR., Whiteman M., Schwarz G., Snyder SH., Paul BD., Carroll KS. and Filipovic MR.




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Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration

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Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration


Participants:

Université de BordeauxInstitut de Biochimie et Génétique Cellulaires. Université de Bordeaux.

Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS).

University of CologneCenter for Molecular Medicine. Institute of Biochemistry. University of Cologne.

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

The Scripps Research InstituteDepartment of Chemistry. The Scripps Research Institute.

University of BelgradeInstitute for Biological Research Sinisa Stankovic. National Institute of Republic of Serbia. University of Belgrade.

Harvard School of Public HealthDepartment of Genetics and Complex Diseases. Harvard School of Public Health.

Technion - Israel Institute of TechnologyRappaport Institute for Research in the Medical Sciences. Faculty of Medicine. Technion - Israel Institute of Technology.

Friedrich-Alexander-Universität Erlangen-NürnbergDepartment of Chemistry and Pharmacy. Friedrich-Alexander-Universität Erlangen-Nürnberg.

University of ExeterMedical School. University of Exeter.

Johns Hopkins University School of MedicineThe Solomon H. Snyder Department of Neuroscience. Johns Hopkins University School of Medicine.

Johns Hopkins University School of MedicineDepartment of Pharmacology and Molecular Sciences. Johns Hopkins University School of Medicine.

Johns Hopkins University School of MedicineDepartment of Psychiatry. Johns Hopkins University School of Medicine.







Cell metabolism
FACTOR YEAR Q
22.415 2018

NLMID: 101233170

PMID: 31735592

ISSN: 1550-4131



TITLE: Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration


JOURNAL: Cell Metab


NUMERACIÓN: S1550-4131(19)30562-5


AÑO: 2020


PUBLISHER: Cell Press


AUTHORS: Zivanovic J., Kouroussis E., Kohl JB., Adhikari B., Bursac B., Schott-Roux S., Petrovic D., Miljkovic JL., Thomas-Lopez D., Jung Y., Miler M., Mitchell S., Milosevic V., Gomes JE., Benhar M., Gonzalez-Zorn B., Ivanovic-Burmazovic I., Torregrossa R., Mitchell JR., Whiteman M., Schwarz G., Snyder SH., Paul BD., Carroll KS. and Filipovic MR.


VISAVET PARTICIPANTS


Bruno González Zorn

DOI: https://doi.org/ 10.1016/j.cmet.2019.10.007


CITE THIS PUBLICATION:

Zivanovic J., Kouroussis E., Kohl JB., Adhikari B., Bursac B., Schott-Roux S., Petrovic D., Miljkovic JL., Thomas-Lopez D., Jung Y., Miler M., Mitchell S., Milosevic V., Gomes JE., Benhar M., Gonzalez-Zorn B., Ivanovic-Burmazovic I., Torregrossa R., Mitchell JR., Whiteman M., Schwarz G., Snyder SH., Paul BD., Carroll KS. and Filipovic MR. Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration. Cell metabolism. S1550-4131(19)30562-5. 2020. (A). ISSN: 1550-4131. DOI: 10.1016/j.cmet.2019.10.007


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