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Sequencing of modern Lepus VDJ genes shows that the usage of VHn genes has been retained in both Oryctolagus and Lepus that diverged 12 million years ago

Investigation article published in Immunogenetics

November 1st, 2013

Among mammals, the European rabbit (Oryctolagus cuniculus) has a unique mechanism of generating the primary antibody repertoire. Despite having over 200 VH genes, the VH1 gene, the most D-proximal VH gene, is used in 80-90 % of VDJ rearrangements, while the remaining 10-20 % is encoded by the VHn genes that map at least 100 Kb upstream of VH1. The maintenance of the VHn genes usage in low frequency in VDJ rearrangements has been suggested to represent a relic of an ancestral immunologic response to pathogens. To address this question, we sequenced VDJ genes for another leporid, genus Lepus, which separated from European rabbit 12 million years ago. Approximately 25 VDJ gene sequences were obtained for each one of three Lepus europaeus individuals. We found that Lepus also uses the VHn genes in 5-10 % of its VDJ rearrangements. Our results show that the VHn genes are a conserved ancestral polymorphism that has been maintained in the leporids genome and is being used for the generation of VDJ rearrangements by both modern Lepus and Oryctolagus.




Pinheiro A., Fernandez de Mera IG., Alves PC., Gortazar C., de la Fuente J. and Esteves PJ.




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Sequencing of modern Lepus VDJ genes shows that the usage of VHn genes has been retained in both Oryctolagus and Lepus that diverged 12 million years ago

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Sequencing of modern Lepus VDJ genes shows that the usage of VHn genes has been retained in both Oryctolagus and Lepus that diverged 12 million years ago



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidade do PortoCentro de Investigação em Biodiversidade e Recursos Genétivos (CIBIO). Universidade do Porto (UP).

Universidade do PortoUniversidade do Porto (UP).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

College of Forestry & Conservation. University of Montana (UMT).

Oklahoma State UniversityDepartament of Veterinary Pathobiology. Center for Veterinary Health Sciences (CVHS). Oklahoma State University (OSU).

Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde (IINFACTS). Cooperativa de Ensino Superior Politécnico e Universitario, CRL (CESPU).







FACTOR YEAR Q
2.488 2013

NLMID: 420404

PMID: 23974323

ISSN: 0093-7711



TITLE: Sequencing of modern Lepus VDJ genes shows that the usage of VHn genes has been retained in both Oryctolagus and Lepus that diverged 12 million years ago


JOURNAL: Immunogenetics


NUMERACIÓN: 65(11):777-84


AÑO: 2013


PUBLISHER: Springer


AUTHORS: Pinheiro A., Fernandez de Mera IG., Alves PC., Gortazar C., de la Fuente J. and Esteves PJ.


DOI: https://doi.org/10.1007/s00251-013-0728-3


CITE THIS PUBLICATION:

Pinheiro A., Fernandez de Mera IG., Alves PC., Gortazar C., de la Fuente J. and Esteves PJ. Sequencing of modern Lepus VDJ genes shows that the usage of VHn genes has been retained in both Oryctolagus and Lepus that diverged 12 million years ago. Immunogenetics. 65(11):777-84. 2013. (A). ISSN: 0093-7711. DOI: 10.1007/s00251-013-0728-3


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