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Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain


April 1st, 2017

Investigation article published in The Journal of Antimicrobial Chemotherapy


OBJECTIVES:
The mobile colistin resistance gene mcr-1 has been identified worldwide in human and animal sources, while its occurrence in the environment is still largely unknown. The aim of this study was to investigate the presence of mcr-1-harbouring Enterobacteriaceae in water samples obtained from rivers and waste water treatment plants in the area of Barcelona, Spain.
METHODS:
The presence of mcr-1 was detected by PCR. Bacterial identification was performed via MALDI-TOF MS. Resistance to colistin was determined by a broth dilution method. The epidemiological relationship between the positive isolates was assessed with PFGE and ST was determined by MLST. Plasmid characterization was performed by transformation experiments, antimicrobial susceptibility testing and incompatibility group PCR.
RESULTS:
Thirty MDR isolates bearing mcr-1, 29 Escherichia coli (ST632 and ST479) and 1 Klebsiella pneumoniae (ST526), were identified in sewage from two different waste water treatment plants, whereas the gene was not found in river water. All isolates, including the K. pneumoniae, harboured blaCTX-M-55 and blaTEM-1 mcr-1 was in all cases associated with an IncI2 plasmid, which only conferred resistance to colistin. mcr-1 was harboured by two predominant E. coli clones that were found in both waste water treatment plants.
CONCLUSIONS:
This study showed a high occurrence of mcr-1 in the sewage of Barcelona, mainly due to the dissemination of two E. coli pulsotypes that are circulating in the population. The presence of mcr-1 in the environment is a cause for concern, and suggests high prevalence of mcr-1 in the community




Ovejero CM., Delgado-Blas JF., Calero-Caceres W., Muniesa M. and Gonzalez-Zorn B..




See this article
Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain

See it on NLM PubMed
Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain

Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universitat de BarcelonaDepartamento de Microbiología. Facultad de Biología. Universitat de Barcelona (UB).







The Journal of Antimicrobial Chemotherapy
FACTOR YEAR Q
5.071 2016

NLMID: 7513617

PMID: 28073965

ISSN: 0305-7453



TITLE: Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain


JOURNAL: J. Antimicrob. Chemother.


NUMERACIÓN: 72(4):1050-1053


AÑO: 2017


PUBLISHER: Oxford University Press


AUTHORS: Ovejero CM., Delgado-Blas JF., Calero-Caceres W., Muniesa M. and Gonzalez-Zorn B..


VISAVET PARTICIPANTS


2nd
José Francisco Delgado Blas
Last
Bruno González Zorn

DOI: https://doi.org/ 10.1093/jac/dkw533


CITE THIS PUBLICATION:

Ovejero CM., Delgado-Blas JF., Calero-Caceres W., Muniesa M. and Gonzalez-Zorn B. Spread of mcr-1-carrying Enterobacteriaceae in sewage water from Spain. The Journal of Antimicrobial Chemotherapy. 72(4):1050-1053. 2017. (A). ISSN: 0305-7453. DOI: 10.1093/jac/dkw533


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