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Successful Host Adaptation of IncK2 Plasmids

Investigation published in Frontiers in Microbiology

October 15th, 2019

The IncK plasmid group can be divided into two separate lineages named IncK1 and IncK2. IncK2 is found predominantly in poultry while IncK1 was reported in various mammals, including animals and humans. The physiological basis of this distinction is not known. In this manuscript we examined fitness cost of IncK1 and IncK2 plasmids at 37 and 42°C, which resembles mammalian and chicken body temperatures, respectively. We analyzed conjugation frequency, plasmid copy number and plasmid fitness cost in direct competition. Additionally, we measured levels of σ-32 in Escherichia coli carrying either wild type or conjugation-deficient IncK plasmids. The results show that IncK2 plasmids have a higher conjugation frequency and lower copy number at 42°C compared to IncK1. While the overall fitness cost to the host bacterium of IncK2 plasmids was higher than that of IncK1, it was not affected by the temperature while the fitness cost of IncK1 was shown to increase at 42°C compared to 37°C. These differences correlate with an increased expression of σ-32, a regulator of heat-shock protein expression, in E. coli with IncK2 compared to cells containing IncK1. This effect was not seen in cells containing conjugation deficient plasmids. Therefore, it is hypothesized that the assembly of the functional T4S may lead to these increased levels of σ-32. Increased activation of CpxR at 42°C may explain why IncK2 plasmids, and not IncK1, are predominantly found in chicken isolates




Rozwandowicz M., Brouwer MSM., Mughini-Gras L., Wagenaar J., Gonzalez-Zorn B., Mevius DJ. and Hordijk J.




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Successful Host Adaptation of IncK2 Plasmids

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Successful Host Adaptation of IncK2 Plasmids


Participants:

Utrecht UniversityDepartment of Infectious Diseases and Immunology. Faculty of Veterinary Medicine. Utrecht University (UU).

Wageningen Bioveterinary Research.

Ministry of Health, Welfare and Sport. Government of the NetherlandsNational Institute for Public Health and the Environment (RIVM). Ministry of Health, Welfare and Sport. Government of the Netherlands.

Utrecht UniversityInstitute for Risk Assessment Sciences. Utrecht University (UU).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Frontiers in Microbiology
FACTOR YEAR Q
4.259 2018

NLMID: 101548977

PMID: 31681238

ISSN: 1664-302X



TITLE: Successful Host Adaptation of IncK2 Plasmids


JOURNAL: Front Microbiol


NUMERACIÓN: 10:2384


AÑO: 2019


PUBLISHER: Frontiers Research Foundation


AUTHORS: Rozwandowicz M., Brouwer MSM., Mughini-Gras L., Wagenaar J., Gonzalez-Zorn B., Mevius DJ. and Hordijk J.


VISAVET PARTICIPANTS


Bruno González Zorn

DOI: https://doi.org/ 10.3389/fmicb.2019.02384


CITE THIS PUBLICATION:

Rozwandowicz M., Brouwer MSM., Mughini-Gras L., Wagenaar J., Gonzalez-Zorn B., Mevius DJ. and Hordijk J. Successful Host Adaptation of IncK2 Plasmids. Frontiers in Microbiology. 10:2384. 2019. (A). ISSN: 1664-302X. DOI: 10.3389/fmicb.2019.02384


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