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The African swine fever virus rp30 ELISA detects antibody in serum and/or oral fluid specimens

Conference in 23 Congreso Internacional de la Sociedad de Veterinarios Porcinos

June 8th, 2014

Gimenez-Lirola LG., Mur L., Rivera B., Wang C., Lizano S., Goodell CK., Rowland R., Harris DL., Gallardo C., Arias M., Sanchez-Vizcaino JM. and Zimmerman JJ.

Introduction
African swine fever virus (ASF) is of major concern because of its high mortality rate, its severe economic impact, and its recent rapid geographic expansion. Prevention and control of ASFV is complicated by the absence of effective vaccines. Serology is useful for ASFV diagnosis and surveillance because antibodies are
a definitive indication of infection and are detectable for
a prolonged period of time (1).
An earlier study showed that ASFV antibodies could be detected in oral fluids (2) and suggested that oral fluids could serve as a suitable specimen for ASFV surveillance. The long-term aim of this line of research is to continue the development of an ASFV antibody ELISA. Several ASFV proteins have been identified as
highly antigenic, e.g., p72, p30 and p54, and others (3). In this study, a recombinant p30 (rp30) polypeptide was selected evaluated in an indirect ELISA format




Participants:

University of Iowa.

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Kansas State UniversityKansas State University (KSU).

Harrisvaccines, Inc.

Centro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Ministerio de Ciencia e Innovación.

Idexx LaboratoriesIdexx Laboratories (IDEXX).


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Publication: The African swine fever virus rp30 ELISA detects antibody in serum and/or oral fluid specimens

Link to 23nd International Pig Veterinary Society Congress





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23nd International Pig Veterinary Society Congress


Jun 8th - September 11st, 2014
Cancún
Mexico

TITLE: The African swine fever virus rp30 ELISA detects antibody in serum and/or oral fluid specimens


TYPE: Oral communication


AUTHORS: Gimenez-Lirola LG., Mur L., Rivera B., Wang C., Lizano S., Goodell CK., Rowland R., Harris DL., Gallardo C., Arias M., Sanchez-Vizcaino JM. and Zimmerman JJ.


VISAVET PARTICIPANTS


3rd
Belén Rivera Arroyo
11th
José Manuel Sánchez-Vizcaíno Rodríguez

DATE: June 8th, 2014


CITE THIS COMMUNICATION:

Gimenez-Lirola LG., Mur L., Rivera B., Wang C., Lizano S., Goodell CK., Rowland R., Harris DL., Gallardo C., Arias M., Sanchez-Vizcaino JM. and Zimmerman JJ. The African swine fever virus rp30 ELISA detects antibody in serum and/or oral fluid specimens. 23nd International Pig Veterinary Society Congress, International Pig Veterinary Society, Cancún, Mexico, June 8th, 2014. (Oral communication)


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