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The testing season affects red deer skinfold increase in response to phytohaemagglutinin

Preventive Veterinary Medicine publish this investigation article

June 1st, 2011

Red deer (Cervus elaphus) have a pronounced seasonality in their physiology. The aim of this study was to assess the effect of the season on red deer responsiveness to skin testing with the phytohaemagglutinin (PHA) mitogen. Study subjects included 270 farmed adult red deer (19 stags and 251 hinds). The skin testing was carried out between January 2009 and August 2010. The animals were injected intradermally with a 0.1ml volume containing 250μg of PHA diluted in phosphate buffered saline. The skinfold thickness was measured immediately prior to injection and 72h after administration, always by the same person and with three repeats per measurement. Single effects of sex and time on skin test responsiveness were significant (p<0.001) as well as their interaction (p<0.001). In winter (January), and considering the average of two years, the skinfold increase in response to the intradermal injection of 250μg PHA was 2.1 times larger in stags and 1.4 times in hinds than in summer (August). While stags had 1.3 times larger responses than hinds in winter, the inverse occurred in summer, with 1.1 times larger responses in hinds. We also evidenced a limited inter-annual variation of skinfold increase in response to PHA in red deer. These findings have important consequences regarding the interpretation of skin test results in the ante-mortem diagnosis of tuberculosis and paratuberculosis, at least in deer




Fernandez de Mera IG., Jaroso R., Martin-Hernando MP., Queiros J., Carta T., Ortiz JA., Vicente J. and Gortazar C.




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The testing season affects red deer skinfold increase in response to phytohaemagglutinin

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The testing season affects red deer skinfold increase in response to phytohaemagglutinin



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidade do PortoCentro de Investigação em Biodiversidade e Recursos Genétivos (CIBIO). Universidade do Porto (UP).

Cinegética Las Lomas.

Universidad Politécnica de MadridEscuela Universitaria de Ingeniería Técnica Agrícola (EUITA). Universidad Politécnica de Madrid (UPM).







Preventive Veterinary Medicine
FACTOR YEAR Q
2.046 2011

NLMID: 8217463

PMID: 21440317

ISSN: 0167-5877



TITLE: The testing season affects red deer skinfold increase in response to phytohaemagglutinin


JOURNAL: Prev. Vet. Med.


NUMERACIÓN: 100(1):79-83


AÑO: 2011


PUBLISHER: Elsevier Science Publishers


AUTHORS: Fernandez de Mera IG., Jaroso R., Martin-Hernando MP., Queiros J., Carta T., Ortiz JA., Vicente J. and Gortazar C.


DOI: https://doi.org/ 10.1016/j.prevetmed.2011.02.011


CITE THIS PUBLICATION:

Fernandez de Mera IG., Jaroso R., Martin-Hernando MP., Queiros J., Carta T., Ortiz JA., Vicente J. and Gortazar C. The testing season affects red deer skinfold increase in response to phytohaemagglutinin. Preventive Veterinary Medicine. 100(1):79-83. 2011. (A). ISSN: 0167-5877. DOI: 10.1016/j.prevetmed.2011.02.011


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