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Trans-species transmission of Brucellae among ruminants hampering brucellosis control efforts in Egypt

Journal of Applied Microbiology publish this investigation article

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This study aimed to identify the genotypic fingerprinting of B. melitensis biovar 3 isolates from ruminants in Kafr El-Sheikh, Egypt, to compare with other peers globally, and to highlight the epidemiology and potential causes of brucellosis control failure.

A multilocus variable-number tandem-repeat analysis (MLVA 16) was carried out on 41 B. melitensis bv3 isolates, 31 from the preferential hosts (28 sheep and three goats), and ten from atypical hosts (nine cattle and one buffalo), identified by bacteriological and molecular techniques. MLVA-16 analysis revealed 19 genotypes with nine as singletons. The most prevalent genotypes were M3_K.E (3,5,3,13,1,1,3,3,7,43,8,7,6,7,5,3), M13_K.E (3,5,3,13,1,1,3,3,7,43,8,5,8,7,7,3), and M5_K.E (3,5,3,13,1,1,3,3,7,43,8,4,8,7,11,3) circulating between different animal species. The B. melitensis isolation from aborted cows in farms that had never reared small ruminants indicates the likelihood of cow to cow B. melitensis transmission. Different genotypes of B. melitensis could be isolated from the same animal. The local geographic distribution of genotypes showed a very close genetic relatedness with genotypes reported outside the study area. Worldwide, our genotypes were mostly related to the Western Mediterranean lineage and less likely to the Americas clonal lineage.

There is a high genetic similarity of B. melitensis bv3 genotypes among different ruminant species and the same animal could be infected with different genotypes. There is a high probability of spreading of B. melitensis among atypical hosts in the absence of the original hosts. The genetic relatedness of B. melitensis bv3 genotypes in the study area with other different geographic areas highlighted the national and international ruminants movement role as a potential factor for maintaining B. melitensis infection.

Further investigations are required to understand the impact of the presence of more than one genotype of B. melitensis in the same animal on the efficacy of brucellosis control strategies.




Hegazy Y., Abdel-Hamid N., Eldahiey M., Oreiby A., Algaabary M., Hamdy M., Beleta E., Martinez I., Shahein M., Garcia N. and Eltholth M.




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Trans-species transmission of Brucellae among ruminants hampering brucellosis control efforts in Egypt

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Trans-species transmission of Brucellae among ruminants hampering brucellosis control efforts in Egypt



Participants:

Kafrelsheikh UniversityAnimal Medicine Department (Infectious diseases). Faculty of Veterinary Medicine. Kafrelsheikh University.

Agricultural Research CenterBrucellosis Research Department. Animal Health Research Institute. Agricultural Research Center.

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Kafrelsheikh UniversityHygiene and Preventive Medicine Department. Faculty of Veterinary Medicine. Kafrelsheikh University.

University of EdinburghGlobal Academy of Agriculture and Food Security. The Royal (Dick) School of Veterinary Studies. University of Edinburgh.







Journal of Applied Microbiology
FACTOR YEAR Q
3.066 2019

NLMID: 9706280

PMID: 34091986

ISSN: 1364-5072



TITLE: Trans-species transmission of Brucellae among ruminants hampering brucellosis control efforts in Egypt


JOURNAL: J. Appl. Microbiol.


PUBLISHER: Wiley


AUTHORS: Hegazy Y., Abdel-Hamid N., Eldahiey M., Oreiby A., Algaabary M., Hamdy M., Beleta E., Martinez I., Shahein M., Garcia N. and Eltholth M.


VISAVET PARTICIPANTS


8th
Irene Martínez Alares
10
Nerea García Benzaquén

DOI: https://doi.org/ https://doi.org/10.1111/jam.15173


CITE THIS PUBLICATION:

Hegazy Y., Abdel-Hamid N., Eldahiey M., Oreiby A., Algaabary M., Hamdy M., Beleta E., Martinez I., Shahein M., Garcia N. and Eltholth M. Trans-species transmission of Brucellae among ruminants hampering brucellosis control efforts in Egypt. Journal of Applied Microbiology. In Press. (A). ISSN: 1364-5072. DOI: https://doi.org/10.1111/jam.15173


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